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AB63376

Anti-Ataxin 1 (phospho S776) antibody

5

(1 리뷰)

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(2 제품이 사용된 논문 )

Rabbit Polyclonal Ataxin 1 phospho S776 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ATXN1 pS776 aa 700 to C-terminus.
1 이미지
Western blot - Anti-Ataxin 1 (phospho S776) antibody (AB63376)
  • WB

Unknown

Western blot - Anti-Ataxin 1 (phospho S776) antibody (AB63376)

All lanes:

Western blot - Anti-Ataxin 1 (phospho S776) antibody (ab63376) at 1/500 dilution

Lane 1:

HepG2 cell extract treated with Adriamycin (0.5 micromoles, 5hours) at 30 µg

Lane 2:

HepG2 cell extract treated with Adriamycin (0.5 micromoles, 5hours) at 30 µg with immunizing phosphopeptide

Predicted band size: 86 kDa

Observed band size: 87 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ATXN1 pS776 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P54253

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대체품

Primary Antibodies

AB201037

Anti-Ataxin 1 antibody [EPR19613]

RabMAb|Recombinant

Immunoprecipitation - Anti-Ataxin 1 antibody [EPR19613] (AB201037)

  • 0
  • 0
Primary Antibodies

AB225895

Anti-Ataxin 1 antibody [EPR19613] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-Ataxin 1 antibody [EPR19613] - BSA and Azide free (AB225895)

  • 0
  • 0
Primary Antibodies

AB186265

Anti-Ataxin 1 antibody [N76/8]

Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 1 antibody [N76/8] (AB186265)

  • 4
  • 1

보완 제품

Primary Antibodies

AB201037

Anti-Ataxin 1 antibody [EPR19613]

RabMAb|Recombinant

Immunoprecipitation - Anti-Ataxin 1 antibody [EPR19613] (AB201037)

  • 0
  • 0
Primary Antibodies

AB284865

Anti-Ataxin 3 antibody [EPR24153-2] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 3 antibody [EPR24153-2] - BSA and Azide free (AB284865)

  • 0
  • 0
Reagents, Controls & Accessories

AB270049

BaseMuncher Endonuclease (TurboNuclease)

RIP - BaseMuncher Endonuclease (TurboNuclease) (AB270049)

  • 0
  • 0
Primary Antibodies

AB126167

Anti-GCSF Receptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody (AB126167)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ataxin-1 sometimes referred to as 'ATXN1' is a protein encoded by the ATXN1 gene. It forms a part of the polyglutamine protein family and typically weighs around 85 kDa. Ataxin-1 appears in various tissues with the highest expression levels in the brain particularly in neurons of the cerebellum and other areas involved in coordination and motor control. This protein consists of multiple domains that contribute to its diverse range of functions including a highly conserved AXH domain involved in protein-protein interactions.
Biological function summary

Ataxin-1 plays a critical role in gene expression regulation and is also implicated in RNA binding. It often associates with large protein complexes such as those involved in RNA metabolism and transcriptional regulation. Ataxin-1 contributes to transcriptional repression through its interaction with transcriptional co-repressors and other proteins involved in gene expression control. This interaction helps maintain normal cellular functions critical for neuronal homeostasis and development.

Pathways

Ataxin-1 interacts with various cellular mechanisms important for normal physiological function. It operates in the Notch signaling pathway influencing cell fate decisions during development. Ataxin-1 is functionally linked to proteins such as RBPJ which cooperates in the regulation of gene expression through these pathways. Additionally it has connections to the Wnt signaling pathway affecting processes that govern cell growth and division alongside beta-catenin an important player in the Wnt signaling pathways.

Ataxin-1 has strong associations with neurodegenerative conditions most notably spinocerebellar ataxia type 1 (SCA1). Variations in the ataxin-1 gene lead to expanded polyglutamine tracts which cause protein misfolding and aggregation contributing to neuronal dysfunction and degeneration. The protein also has potential links to Alzheimer's disease where altered ataxin-1 expression and interaction with amyloid precursor protein (APP) may exacerbate disease progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism (PubMed : 21475249). In concert with CIC and ATXN1L, involved in brain development (By similarity).
See full target information ATXN1 pS776

대체 명칭 보기

ATX1, SCA1, ATXN1, Ataxin-1, Spinocerebellar ataxia type 1 protein
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Proceedings of the National Academy of Sciences of 115:E12407-E12416 PubMed30530649

2018

MTSS1/Src family kinase dysregulation underlies multiple inherited ataxias.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander S Brown,Pratap Meera,Banu Altindag,Ravi Chopra,Emma M Perkins,Sharan Paul,Daniel R Scoles,Eric Tarapore,Jessica Magri,Haoran Huang,Mandy Jackson,Vikram G Shakkottai,Thomas S Otis,Stefan M Pulst,Scott X Atwood,Anthony E Oro

Journal of neurochemistry 114:706-16 PubMed20477910

2010

Dopamine D2 receptor signaling modulates mutant ataxin-1 S776 phosphorylation and aggregation.

Applications

ICC/IF

Species

Human

Scoty M Hearst,Mariper E Lopez,Qingmei Shao,Yong Liu,Parminder J S Vig
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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