Anti-Ataxin 7 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 7 antibody (AB11434)

ICC/IF image of ab11434 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11434, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 7 antibody (AB11434)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized, 70% confluent log phase U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells, treated with 100 ng of Nocodazole for 16 hours. Labelling Ataxin 7 with ab11434 at 2 μg/mL and incubated for 3 hours at room temperature and then labelled with Goat anti-Rabbit IgG secondary antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Green). Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (Blue). F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e is untreated cell with nuclear localization. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

• WB

Unknown

Western blot - Anti-Ataxin 7 antibody (AB11434)

Western blot of Ataxin 7 in transfected COS-1 cell lysate using ab11434.

All lanes:

Western blot - Anti-Ataxin 7 antibody (ab11434)

Predicted band size: 95 kDa

false