Anti-ATF6 antibody

• ICC

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Immunocytochemistry - Anti-ATF6 antibody (AB174756)

Immunocytochemistry analysis of paraformaldehyde fixed A431 cells labelling ATF6 with ab174756 at 10 μg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL). The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL).

• ICC

Supplier Data

Immunocytochemistry - Anti-ATF6 antibody (AB174756)

Immunocytochemistry analysis of paraformaldehyde fixed HeLa cells labelling ATF6 with ab174756 at 10 μg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL). The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL).

• WB

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Western blot - Anti-ATF6 antibody (AB174756)

All lanes:

Western blot - Anti-ATF6 antibody (ab174756) at 1 µg/mL

Lane 1:

HeLa whole cell lysate in RIPA buffer at 35 µg

Lane 2:

MCF7 whole cell lysate in RIPA buffer at 35 µg

Lane 3:

HeLa nuclear cell lysate in RIPA buffer at 35 µg

Lane 4:

Jurkat nuclear cell lysate in RIPA buffer at 35 µg

Predicted band size: 74 kDa

Observed band size: 75-80 kDa

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