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AB205302

Anti-ATHL1 antibody [EPR13421]

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Rabbit Recombinant Monoclonal ATHL1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
4 이미지
Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (AB205302)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (AB205302)

Immunofluorescent analysis of 100% Methanol-fixed 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ATHL1 with ab205302 at 1/250 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on MCF-7 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab205302 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (AB205302)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (AB205302)

Immunofluorescent analysis of 100% Methanol-fixed 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling ATHL1 with ab205302 at 1/250 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on BxPC-3 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab205302 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Western blot - Anti-ATHL1 antibody [EPR13421] (AB205302)
  • WB

Supplier Data

Western blot - Anti-ATHL1 antibody [EPR13421] (AB205302)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATHL1 antibody [EPR13421] (ab205302) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 10 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 81 kDa

Observed band size: 80 kDa

false

Exposure time: 1min

Western blot - Anti-ATHL1 antibody [EPR13421] (AB205302)
  • WB

Supplier Data

Western blot - Anti-ATHL1 antibody [EPR13421] (AB205302)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATHL1 antibody [EPR13421] (ab205302) at 1/1000 dilution

All lanes:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 81 kDa

Observed band size: 80 kDa

false

Exposure time: 3min

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-ATHL1 antibody [EPR13421] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13421

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB154123

Anti-SH3YL1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3YL1 antibody (AB154123)

  • 0
  • 0
Primary Antibodies

AB204583

Anti-Keratinocyte differentiation associated protein antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratinocyte differentiation associated protein antibody (AB204583)

  • 0
  • 0
Assay Kits

AB215539

Human IL-18 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-18 ELISA Kit (AB215539)

  • 5
  • 1
Primary Antibodies

AB217330

Anti-Transmembrane protein 30A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane protein 30A antibody (AB217330)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ATHL1 also known as ATHL1 protein is a protein with a molecular weight of approximately 70 kDa. Known to be expressed largely in the human brain ATHL1 plays a role in various cellular functions. It is found distributed in other tissues as well but with lower levels noted. This protein interacts with cellular pathways and processes helping in the regulation of key molecular functions.
Biological function summary

ATHL1 engages with cytoskeletal elements and impact cellular structure and stability. It does not form part of a larger protein complex but acts through a collaborative network with other proteins. Its interactions support various cellular processes by influencing the components involved in maintaining cellular shape and integrity. ATHL1's role in these processes makes it an important element in the understanding of cellular dynamics.

Pathways

ATHL1 gets involved in the regulation of the MAPK/ERK pathway. It links with critical components like ERK1/2 affecting cell growth and survival. Another significant pathway involving ATHL1 is the Wnt signaling pathway. This pathway important for numerous developmental processes sees ATHL1 contributing to cellular signaling cascades that determine cell fate and proliferation.

ATHL1 has links to neurological conditions notably Alzheimer's disease. It potentially interacts with tau protein which is known for its role in neurodegenerative processes. Additionally ATHL1's involvement in cellular structural integrity suggests potential ties to cancer where disruptions in cell framework can lead to malignant transformations. Its connections with cytoskeletal proteins highlight ATHL1's role in disease mechanisms and offer avenues for therapeutic exploration.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalyzes the hydrolysis of glucose from the disaccharide unit linked to hydroxylysine residues of collagen and collagen-like proteins.
See full target information PGGHG

대체 명칭 보기

ATHL1, PGGHG, Protein-glucosylgalactosylhydroxylysine glucosidase, Acid trehalase-like protein 1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

Cell communication and signaling : CCS 23:412 PubMed41039499

2025

A comparative proteomic, transcriptomic and glycomic analysis of extracellular vesicle isolation techniques highlights ExoGAG efficiency for a more complete identification of breast milk molecular signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

María Pereira-Hernández,Rosaura Picáns-Leis,María E Vázquez-Mosquera,Nerea Lago-Baameiro,Pedro Fortes-González,Laura López-Valverde,Ana Barcia de la Iglesia,Laura Núñez-González,Susana B Bravo,María Pardo,María L Couce,Miguel A Garcia-Gonzalez
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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