AB190875

Anti-ATP5C1 antibody

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Goat Polyclonal ATP5C1 antibody. Suitable for WB and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATP5F1C aa 1-50.

대체 명칭 보기

ATP5C, ATP5C1, ATP5CL1, ATP5F1C, ATP synthase F1 subunit gamma, F-ATPase gamma subunit

1 이미지

Supplier Data

Western blot - Anti-ATP5C1 antibody (AB190875)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-ATP5C1 antibody (ab190875) at 0.1 µg/mL

All lanes:

Rat skeletal muscle lysate (in RIPA buffer) at 35 µg

Predicted band size: 33 kDa

Observed band size: 30 kDa

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주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Synthetic Peptide within Human ATP5F1C aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P36542

Specificity

ab190875 is expected to recognize both reported isoforms (NP_005165.1; NP_001001973.1). The immunizing peptide represents the N terminus of the mature protein.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab190875 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The ATP5C1 protein also known as ATP synthase subunit gamma plays an important role in cellular energy production. This protein has a mass of approximately 55 kDa. It remains primarily expressed in the mitochondria the powerhouse of cells where it forms a part of the ATP synthase complex. ATP5C1 is essential for ATP synthesis a pivotal process for energy production in cells.

Biological function summary

The ATP5C1 protein is an integral component of the F1 sector of the ATP synthase complex. This complex transduces electrochemical energy into ATP which is the main energy carrier in biological systems. ATP5C1 facilitates rotation of the complex’s central stalk enabling energy conversion. It ensures ATP is produced efficiently supporting various cellular processes. ATP5C1 expression levels affect cellular metabolism and energy homeostasis.

Pathways

ATP5C1 holds an important position within oxidative phosphorylation and the mitochondrial electron transport chain. These pathways are vital for ATP production and involve several proteins and enzymes including NADH dehydrogenase cytochrome c oxidase and other ATP synthase subunits. ATP5C1 interacts with these proteins to sustain the flow of electrons coupling it with proton gradients that drive ATP synthesis.

ATP5C1 mutations or malfunctions link to conditions such as mitochondrial diseases and metabolic disorders. For instance alterations in ATP5C1 can affect ATP synthesis efficiency leading to mitochondrial dysfunction. It also associates with neurodegenerative diseases where inefficient energy production exacerbates neuronal degradation. The protein's dysfunction may involve other proteins like ATP5D a fellow subunit within the ATP synthase complex that collaborates in maintaining ATP production.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Subunit gamma, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). With the central stalk subunit delta, is essential for the biogenesis of F(1) catalytic part of the ATP synthase complex namely in the formation of F1 assembly intermediate (PubMed : 29499186).

See full target information ATP5F1C

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 4:775 PubMed34163008

2021

Cyanocobalamin prevents cardiomyopathy in type 1 diabetes by modulating oxidative stress and DNMT-SOCS1/3-IGF-1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Masao Kakoki,Purushotham V Ramanathan,John R Hagaman,Ruriko Grant,Jennifer C Wilder,Joan M Taylor,J Charles Jennette,Oliver Smithies,Nobuyo Maeda-Smithies

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP5C1 antibody

Western blot - Anti-ATP5C1 antibody (AB190875)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise