AB217054

Anti-ATP5F1 antibody

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Rabbit Polyclonal ATP5F1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATP5PB aa 1-100.

대체 명칭 보기

ATP5F1, ATP5PB, ATP synthase peripheral stalk-membrane subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase subunit b, ATPase subunit b

2 이미지

Supplier Data

Immunoprecipitation - Anti-ATP5F1 antibody (AB217054)

Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling ATP5F1 using ab217054 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated ATP5F1, ab217054 was used at 1 μg/ml. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ATP5F1 antibody (ab217054)

Predicted band size: 29 kDa

false

Supplier Data

Western blot - Anti-ATP5F1 antibody (AB217054)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-ATP5F1 antibody (ab217054) at 0.1 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

293T cell lysate at 50 µg

Lane 3:

Jurkat cell lysate at 50 µg

Lane 4:

TCMK1 cell lysate at 50 µg

Lane 5:

NIH 3T3 cell lysate at 50 µg

Predicted band size: 29 kDa

true

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ATP5PB aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P24539

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab217054 was affinity purified using an epitope specific to ATP5F1 immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ATP5F1 also known as ATP synthase F(0) complex subunit B1 is a protein found in the inner mitochondrial membrane. It is a component of the ATP synthase enzyme an important player in energy production with a molecular weight around 55 kDa. ATP5F1 is expressed abundantly in energy-demanding tissues such as the heart liver and skeletal muscle. This protein plays an important mechanical role in synthesizing adenosine triphosphate (ATP) by catalyzing the combination of adenosine diphosphate (ADP) and inorganic phosphate.

Biological function summary

ATP5F1 serves as an important part of the ATP synthase complex which consists of various subunits forming the F1 and Fo regions. This complex carries out the final step of oxidative phosphorylation in the mitochondria by using the proton gradient across the mitochondrial membrane to drive ATP production. As a critical process in cellular respiration ATP generation influences numerous cellular functions including muscle contraction nerve impulse propagation and synthesis of biomolecules.

Pathways

ATP5F1 operates within the oxidative phosphorylation and electron transport chain pathways. These pathways are important for maintaining the cell's energy balance. ATP5F1 works closely with other proteins such as ATP synthase Fo subunits aiding in proton translocation and ensuring efficient ATP synthesis. Disruptions in these pathways can have significant effects on cellular metabolism and energy homeostasis.

ATP5F1 is linked to mitochondrial dysfunctions such as mitochondrial myopathy and Leigh syndrome. These conditions result from impaired oxidative phosphorylation leading to insufficient ATP production. ATP5F1 interacts with the NADH dehydrogenase complex and mutations can affect their functionality contributing to these disorders. Understanding ATP5F1's role helps researchers explore therapeutic strategies aimed at managing these mitochondrial diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). Part of the complex F(0) domain (PubMed : 37244256). Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements (By similarity).

See full target information ATP5PB

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Human cell 36:812-821 PubMed36629984

2023

SPOCK2 and SPRED1 function downstream of EZH2 to impede the malignant progression of lung adenocarcinoma in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Xiaoxi Fan,Changrui Jiang,Shun Xu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP5F1 antibody

Immunoprecipitation - Anti-ATP5F1 antibody (AB217054)

Western blot - Anti-ATP5F1 antibody (AB217054)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise