Anti-ATP5H antibody

• IP

Supplier Data

Immunoprecipitation - Anti-ATP5H antibody (AB241164)

ATP5H was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241164 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241164 at 0.4 μg/ml.

Lane 1 : ab241164 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

Lysate prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-ATP5H antibody (ab241164)

Predicted band size: 18 kDa,37 kDa

false

• WB

Supplier Data

Western blot - Anti-ATP5H antibody (AB241164)

Lysates were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-ATP5H antibody (ab241164) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) cell lysate at 15 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 15 µg

Predicted band size: 18 kDa

true

Exposure time: 10s