AB236375

Anti-ATP6V0D2 antibody [OTI2D6]

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(7 제품이 사용된 논문 )

Mouse Monoclonal ATP6V0D2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ATP6V0D2.

대체 명칭 보기

V-type proton ATPase subunit d 2, V-ATPase subunit d 2, Vacuolar proton pump subunit d 2, ATP6V0D2

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D2 antibody [OTI2D6] (AB236375)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D2 antibody [OTI2D6] (AB236375)

Paraffin-embedded human kidney tissue stained for ATP6V0D2 with ab236375 at a 1/2000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D2 antibody [OTI2D6] (AB236375)

Paraffin-embedded human pancreas tissue stained for ATP6V0D2 with ab236375 at a 1/2000 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ATP6V0D2 antibody [OTI2D6] (AB236375)

All lanes:

Western blot - Anti-ATP6V0D2 antibody [OTI2D6] (ab236375) at 1/500 dilution

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) cell extracts

Predicted band size: 40 kDa

false

Supplier Data

Western blot - Anti-ATP6V0D2 antibody [OTI2D6] (AB236375)

All lanes:

Western blot - Anti-ATP6V0D2 antibody [OTI2D6] (ab236375) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY ATP6V0D2 cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Predicted band size: 40 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2D6

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ATP6V0D2.

Q8N8Y2

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p>Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

Purified from cell culture supernatant.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V0D2 also known as V-type proton ATPase subunit d 2 is a subunit of the V-ATPase complex. It has a mass of approximately 38 kDa. This protein is expressed in various tissues with significant expression noted in bone and white adipose tissue. Mechanically ATP6V0D2 contributes to the acidification of intracellular compartments. It functions as part of the proton pump machinery facilitating the transfer of protons across membranes which is essential for processes like endocytosis and intracellular trafficking.

Biological function summary

ATP6V0D2 plays a significant role in bone resorption and adipogenesis. It is a component of the larger V-ATPase complex important for creating acidic environments required for enzyme activity particularly in osteoclasts. This acidity enables the breakdown of bone matrix which is a fundamental part of bone remodeling. Additionally ATP6V0D2 influences fat cell differentiation by modulating the function of cytosolic components within the complex.

Pathways

ATP6V0D2 functions as an integral part of the bone remodeling and lysosomal degradation pathways. In the bone remodeling pathway the protein works closely with other proton pump components like ATP6V0A3 to regulate osteoclast activity. In lysosomal degradation it aids in maintaining the acidity essential for lysosomal enzyme activity. Both pathways are connected through ATP6V0D2's regulation of intracellular pH and ion homeostasis.

ATP6V0D2 has associations with osteoporosis and obesity. In osteoporosis deficient or dysfunctional ATP6V0D2 activity can impair osteoclast function leading to altered bone resorption and skeletal fragility. In the context of obesity this protein influences adipocyte differentiation and function. ATP6V0D2's role in these disorders connects it to other proteins like RANKL in bone metabolism and PPARγ in adipogenesis linking it beyond just its structural function within proton pumps.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). May play a role in coupling of proton transport and ATP hydrolysis (By similarity). Regulator of osteoclast fusion and bone formation (By similarity).

See full target information ATP6V0D2

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of translational medicine 23:805 PubMed40682116

2025

Sinensetin serves as an AMPK activator to inhibit RANKL-induced osteoclastogenesis via osteoclast cytoskeleton reorganization.

Applications

Unspecified application

Species

Unspecified reactive species

Yijie Gao,Rui Zhou,Xixi Lin,Zhijuan Liu,Yuangang Su,Haoyu Lian,Bin Lin,Xiaofei Ding,Shijie Liao,Xiangde Li,Jinmin Zhao,Jiake Xu,Ren Xu,Qian Liu,Fangming Song

OncoTargets and therapy 17:1209-1222 PubMed39735788

2024

Development of a Prognostic Risk Model for Esophageal Cancer Based on M0 Macrophage-Related Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Zuo,Fuqiang Wang,Guofeng Liu,Shenglong Xie,Senyi Deng,Yun Wang

Phytotherapy research : PTR 38:1971-1989 PubMed38358727

2024

Cichoric acid targets RANKL to inhibit osteoclastogenesis and prevent ovariectomy-induced bone loss.

Applications

Unspecified application

Species

Unspecified reactive species

Yansi Xian,Yijie Gao,Yiji Su,Yuangang Su,Haoyu Lian,Xiaoliang Feng,Zhijuan Liu,Jinmin Zhao,Jiake Xu,Qian Liu,Fangming Song

Journal of translational medicine 21:839 PubMed37993937

2023

Myrislignan targets extracellular signal-regulated kinase (ERK) and modulates mitochondrial function to dampen osteoclastogenesis and ovariectomy-induced osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Yang,Weiwei Chen,Kai Gan,Chaofeng Wang,Xiaoxiao Xie,Yuangang Su,Haoyu Lian,Jiake Xu,Jinmin Zhao,Qian Liu

iScience 26:107760 PubMed37720109

2023

CGK733 alleviates ovariectomy-induced bone loss through blocking RANKL-mediated Ca oscillations and NF-κB/MAPK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Minglian Xu,Dezhi Song,Xiaoxiao Xie,Yiwu Qin,Jian Huang,Chaofeng Wang,Junchun Chen,Yuangang Su,Jiake Xu,Jinmin Zhao,Qian Liu

Genes 14: PubMed36980869

2023

Distribution and Expression of Pulmonary Ionocyte-Related Factors CFTR, ATP6V0D2, and ATP6V1C2 in the Lungs of Yaks at Different Ages.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng He,Yating Wei,Yan Cui,Qian Zhang

Frontiers in pharmacology 13:829862 PubMed35345816

2022

CYT387, a JAK-Specific Inhibitor Impedes Osteoclast Activity and Oophorectomy-Induced Osteoporosis Modulating RANKL and ROS Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Jiamin Liang,Liwei Wu,Yang Xu,Chengxiang Xiao,Xue Yang,Ran Sun,Jinmin Zhao,Jiake Xu,Qian Liu,Bo Zhou

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP6V0D2 antibody [OTI2D6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D2 antibody [OTI2D6] (AB236375)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D2 antibody [OTI2D6] (AB236375)

Western blot - Anti-ATP6V0D2 antibody [OTI2D6] (AB236375)

Western blot - Anti-ATP6V0D2 antibody [OTI2D6] (AB236375)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (7)

Product promise