AB254685

Anti-ATP6V1H antibody

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Rabbit Polyclonal ATP6V1H antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V1H aa 400-500.

대체 명칭 보기

CGI-11, ATP6V1H, V-type proton ATPase subunit H, V-ATPase subunit H, Nef-binding protein 1, Protein VMA13 homolog, V-ATPase 50/57 kDa subunits, Vacuolar proton pump subunit H, Vacuolar proton pump subunit SFD, NBP1

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (AB254685)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (AB254685)

Paraffin-embedded human fallopian tube tissue stained for ATP6V1H using ab254685 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1H antibody (AB254685)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1H antibody (AB254685)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling ATP6V1H using ab254685 at 4µg/ml (green) in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (AB254685)

Paraffin-embedded human prostate tissue stained for ATP6V1H using ab254685 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (AB254685)

Paraffin-embedded human kidney tissue stained for ATP6V1H using ab254685 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (AB254685)

Paraffin-embedded human cerebral cortex tissue stained for ATP6V1H using ab254685 at 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ATP6V1H antibody (AB254685)

All lanes:

Western blot - Anti-ATP6V1H antibody (ab254685) at 0.4 µg/mL

Lane 1:

Vector only transfected HEL-293T cell lysate

Lane 2:

HEK-293T overexpressing ATP6V1H with a C-terminal myc-DDK tag, cell lysate

Predicted band size: 56 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ATP6V1H aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

Q9UI12

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V1H also known as VMA13 or V1H is a subunit of the V1 domain in vacuolar ATPases (V-ATPases). It plays a role in the formation of proton channels important for acidifying vesicular environments. The protein has a mass of approximately 54 kDa. The gene encoding ATP6V1H is expressed in various tissues with higher levels observed in the kidney and brain indicating a more demanding need for ion transport and homeostasis in these tissues.

Biological function summary

ATP6V1H functions within the V-ATPase complex which is essential for acidifying intracellular organelles such as lysosomes and endosomes. This subunit contributes to energy-dependent proton transport affecting pH regulation within cells and intracellular processes like protein degradation and receptor-mediated endocytosis. It often cooperates with other subunits within the V-ATPase complex to maintain proper cell function and homeostasis.

Pathways

ATP6V1H participates in critical processes such as endocytosis and protein sorting pathways. These pathways influence cellular environment and nutrient sensing. The ATP6V1H subunit interacts with proteins like ATP6V1A and ATP6V0C which are other components of the V-ATPase complex. Together they form a complex vital for membrane-mediated transport and cellular signaling pathways.

ATP6V1H has been linked to conditions like osteoporosis and neurodegenerative diseases. The protein's malfunction can lead to improper acidification within the cell affecting bone resorption and neural activity. Disorders related to ATP6V1H often involve mutations in the associated V-ATPase complex subunits such as ATP6V1B1. These dysfunctional pathways highlight the importance of ATP6V1H in maintaining normal cellular and physiological functions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). Subunit H is essential for V-ATPase activity, but not for the assembly of the complex (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes (PubMed : 12032142).

See full target information ATP6V1H

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 25: PubMed38542224

2024

Mitochondrial Ribosomal Protein MRPS15 Is a Component of Cytosolic Ribosomes and Regulates Translation in Stressed Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Florian David,Emilie Roussel,Carine Froment,Tangra Draia-Nicolau,Françoise Pujol,Odile Burlet-Schiltz,Anthony K Henras,Eric Lacazette,Florent Morfoisse,Florence Tatin,Jean-Jacques Diaz,Frédéric Catez,Barbara Garmy-Susini,Anne-Catherine Prats

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP6V1H antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (AB254685)

Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1H antibody (AB254685)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (AB254685)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (AB254685)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (AB254685)

Western blot - Anti-ATP6V1H antibody (AB254685)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise