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AB240880

Anti-ATP7b antibody [S62-29] - N-terminal

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Mouse Monoclonal ATP7B antibody. N-terminal. Suitable for ICC/IF and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATP7B aa 1-50.

대체 명칭 보기

PWD, WC1, WND, ATP7B, Copper-transporting ATPase 2, Copper pump 2, Wilson disease-associated protein

1 이미지
Immunocytochemistry/ Immunofluorescence - Anti-ATP7b antibody [S62-29] - N-terminal (AB240880)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATP7b antibody [S62-29] - N-terminal (AB240880)

4% Formaldehyde-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells stained for ATP7b (Green) using ab240880 at 1/100 dilution for 60 minutes at RT. The secondary antibody used was a Goat Anti-Mouse ATTO 488 at 1 : 200 for 60 min at RT. Phalloidin Texas Red F-Actin stain was used as a counterstain (Red) at 1/1000 dilution for 60 minutes at RT. The nuclear counterstain was DAPI (Blue) at 1/5000 dilution for 5 minutes at RT.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

S62-29

Isotype

IgG1

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human ATP7B aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P35670

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대체품

Primary Antibodies

AB262897

Anti-ATP7b antibody

Immunocytochemistry/ Immunofluorescence - Anti-ATP7b antibody (AB262897)

  • 0
  • 0
Primary Antibodies

AB217299

Anti-ATP7b antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP7b antibody - C-terminal (AB217299)

  • 0
  • 0
Primary Antibodies

AB211985

PE Anti-ATP7b antibody [EPR6794]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-ATP7b antibody [EPR6794] (AB211985)

  • 0
  • 0
Primary Antibodies

AB124973

Anti-ATP7b antibody [EPR6794]

20ul selling size|KO Validated|RabMAb|Recombinant

Western blot - Anti-ATP7b antibody [EPR6794] (AB124973)

  • 1
  • 1
Primary Antibodies

AB240049

Anti-ATP7b antibody [EPR6793] - BSA and Azide free

RabMAb|Recombinant

OI-RD Scanning - Anti-ATP7b antibody [EPR6793] - BSA and Azide free (AB240049)

  • 0
  • 0
Primary Antibodies

AB240011

Anti-ATP7b antibody [EPR6794] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ATP7b antibody [EPR6794] - BSA and Azide free (AB240011)

  • 0
  • 0
Primary Antibodies

AB131208

Anti-ATP7b antibody [EPR6793]

RabMAb|Recombinant

Western blot - Anti-ATP7b antibody [EPR6793] (AB131208)

  • 4
  • 1
Primary Antibodies

AB133731

Anti-ATP7b antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP7b antibody (AB133731)

  • 0
  • 0

보완 제품

Assay Kits

AB150666

Copper Stain Kit

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Copper Stain Kit (AB150666)

  • 0
  • 0
Primary Antibodies

AB317432

Anti-SLC31A1 / CTR1 antibody [S06-7G0]

Recombinant

  • 0
  • 0
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13
Primary Antibodies

AB46545

Anti-4 Hydroxynonenal antibody

Western blot - Anti-4 Hydroxynonenal antibody (AB46545)

  • 4
  • 27
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purified from TCS.
보관 버퍼
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ATP7B also known as Wilson disease protein is a copper-transporting ATPase. This protein has a mass of approximately 146 kDa. It is mainly expressed in the liver kidney placenta and brain. ATP7B functions mechanically by transporting copper across different cellular compartments. It utilizes ATP hydrolysis to pump copper ions helping the body regulate copper homeostasis. This transport activity is located predominantly in the trans-Golgi network where ATP7B assists in incorporating copper into ceruloplasmin a critical copper-carrying blood plasma protein.
Biological function summary

ATP7B plays a significant role in maintaining copper balance within the body. It associates with intracellular vesicles and through its catalytic activity influences various cellular metabolic processes. Although ATP7B does not typically form large complexes its interaction with other proteins and cellular organelles contributes to copper ion binding and transfer. Proper ATP7B function ensures that copper is channeled effectively to places where it is required for enzymatic activity or is expelled from cells to prevent accumulation.

Pathways

ATP7B is a central figure in the copper transport and homeostasis pathway. Its role is directly connected to the biosynthesis of copper-dependent enzymes. The protein impacts the pathway involving ceruloplasmin biosynthesis by regulating copper ion incorporation. ATP7A another copper-transporting ATPase shares similar pathway responsibilities but in different tissues showing divergence in their specific biological roles. Together they ensure whole-body copper balance.

ATP7B mutations or dysfunction is strongly implicated in Wilson's disease a genetic disorder characterized by excessive copper accumulation. This can result in hepatic neurological and psychiatric symptoms due to copper buildup. The protein's relationship to Wilson's disease suggests its critical role in preventing copper toxicity. There is also evidence linking ATP7B with Menkes disease but ATP7A plays a more direct role in Menkes highlighting the distinct responsibilities these ATPases have in different tissues and conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload.
See full target information ATP7B
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Kaohsiung journal of medical sciences 39:916-926 PubMed37338034

2023

Suppression of cAMP/PKA/CREB signaling ameliorates retinal injury in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Ling Fang,Qin Zhang,Wen-Wen Xue,Jin-Hua Tao,Hai-Dong Zou,Qiu-Rong Lin,Yu-Lan Wang
제품이 사용된 논문 모두 보기
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Product promise

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