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AB74235

Anti-ATPAF2 antibody

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Rabbit Polyclonal ATPAF2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATPAF2.
1 이미지
Western blot - Anti-ATPAF2 antibody (AB74235)
  • WB

Unknown

Western blot - Anti-ATPAF2 antibody (AB74235)

All lanes:

Western blot - Anti-ATPAF2 antibody (ab74235) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells at 5 µg

Lane 2:

Extracts from Jurkat cells plus 5µg immunizing peptide at 5 µg

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ATPAF2. The exact immunogen used to generate this antibody is proprietary information.

Q8N5M1

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보완 제품

Primary Antibodies

AB155999

Anti-SDHC antibody [EPR11035(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (AB155999)

  • 0
  • 0
Primary Antibodies

AB198196

Anti-NDUFA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA2 antibody (AB198196)

  • 0
  • 0
Reagents, Controls & Accessories

AB110413

Total OXPHOS Rodent WB Antibody Cocktail

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

  • 5
  • 18
Primary Antibodies

AB110242

Anti-NDUFB8 antibody [20E9DH10C12]

Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (AB110242)

  • 5
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The ATPAF2 protein also known as ATP synthase mitochondrial F1 complex assembly factor 2 plays a significant role in the mitochondrial ATP synthase assembly. It is approximately 33 kDa in size. This protein is expressed mainly in tissues with high-energy demands such as muscle and brain tissue. It aids in the proper assembly of the ATP synthase complex by supporting the integration of certain subunits which is vital for maintaining mitochondrial function and energy production.
Biological function summary

ATPAF2 assists in the proper assembly of proton-translocating ATP synthase complexes within mitochondria. It is not directly a part of the ATP synthase complex but acts as an assembly factor that facilitates the inclusion of F1 complex components. This participation is important for the generation of ATP via oxidative phosphorylation—a process pivotal for cellular energy management.

Pathways

ATPAF2 is essential for the efficient functioning of the oxidative phosphorylation pathway. This pathway is responsible for ATP production in cells via the mitochondrial electron transport chain. Within this pathway ATPAF2 relates closely with ATP5B and ATP5C1 proteins which form integral parts of the ATP synthase complex. These interactions help ensure that ATP synthase is constructed correctly enabling the efficient synthesis of ATP.

ATPAF2 mutations have been associated with mitochondrial diseases and neurodegenerative disorders. Mitochondrial abnormalities caused by improper ATP synthase assembly can lead to conditions like Leigh syndrome. Proteins like ATP5A1 and ATP5O which also participate in the biogenesis of ATP synthase can demonstrate interconnected malfunction when ATPAF2 is impaired contributing to the severity of these diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Plays a role in the assembly of the F1 component of the mitochondrial ATP synthase (ATPase).
See full target information ATPAF2

대체 명칭 보기

ATP12, LP3663, ATPAF2, ATP synthase mitochondrial F1 complex assembly factor 2, ATP12 homolog
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

NPJ systems biology and applications 11:67 PubMed40593928

2025

Temporal analysis of doxorubicin-induced cardiac toxicity and hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Te Lin,Yi-Ju Lee,Wen-Wei Tseng,Zih-Hua Chen,Huai-Ching Hsieh,Ko-Hong Lin,Jin-Yu Su,An-Chi Wei
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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