AB223779

Anti-ATPase Inhibitory Factor 1/IF1 antibody

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Rabbit Polyclonal ATPase Inhibitory Factor 1/IF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP5IF1 aa 1-150.

대체 명칭 보기

ATPI, ATPIF1, ATP5IF1, ATP synthase F1 subunit epsilon, Inhibitor of F(1)F(o)-ATPase, IF(1), IF1

7 이미지

Immunocytochemistry/ Immunofluorescence - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ATPase Inhibitory Factor 1/IF1 (green) using ab223779 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Paraffin-embedded human kidney tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Paraffin-embedded human fallopian tube tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Paraffin-embedded human duodenum tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Paraffin-embedded human testis tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Paraffin-embedded human rectum tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

All lanes:

Western blot - Anti-ATPase Inhibitory Factor 1/IF1 antibody (ab223779) at 0.4 µg/mL

All lanes:

Human heart tissue

Predicted band size: 12 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ATP5IF1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9UII2

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ATPase Inhibitory Factor 1 (IF1) also referred to as IF1 protein ATPIF1 or ATPase inhibitory factor 1 is a small protein with a mass of approximately 12 kDa. It is expressed primarily in mitochondria-rich tissues such as the heart liver and brain where it plays an important role in energy metabolism. IF1 is notable for its ability to bind to the F1 subunit of the mitochondrial ATP synthase inhibiting its ATPase activity under specific conditions.

Biological function summary

IF1 influences mitochondrial function by regulating ATP synthase in response to changes in the cellular environment. Under conditions of low cellular energy IF1 stabilizes and prevents hydrolysis of ATP by binding to the F1 catalytic domain of the enzyme. This regulation helps to conserve ATP critical for maintaining cellular viability during stress. IF1 can also interact with other mitochondrial proteins to form complexes that influence energy homeostasis in the cell.

Pathways

IF1 integrates into pathways involved in cellular energy management including oxidative phosphorylation and ATP synthesis pathways. It interacts significantly with proteins such as the mitochondrial F1Fo-ATP synthase complex. By modulating ATP synthase activity IF1 ensures efficient ATP production and maintains mitochondrial membrane potential important for proper cell function.

IF1 has associations with metabolic conditions and cancer. Abnormal expression or activity of IF1 correlates with mitochondrial dysfunction often observed in cancer cells where it affects cell proliferation. Additionally IF1 has links to cardiac conditions given its role in regulating heart metabolism. It relates to proteins like the cytochrome c oxidase influencing cellular respiration and affecting disease progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix (PubMed : 35926043). Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues : regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.

See full target information ATP5IF1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal for immunotherapy of cancer 13: PubMed40954077

2025

LncRNA-CTD suppresses metastasis and immune evasion by modulating snail1 and MHC-I expression in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Xu,Huisi Qiu,Yuezhang Sun,Hong Jin,Siyu Yan,Fangmei Zhang,Ying Song,Guopei Zheng,Dongjiang Liao,Xiaoli Wei,Liyun Luo

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATPase Inhibitory Factor 1/IF1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Western blot - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise