Anti-ATRX antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody (AB204169)

Immunohistochemical analysis of human liver tissue labeling ATRX in the hepatocytes with ab204169 at a 1/500 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATRX antibody (AB204169)

Immunocytochemical analysis of A549 (Human lung carcinoma cell line) whole cells labeling ATRX in the nucleoplasm and nuclear bodies with ab204169 at 2 μg/ml.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody (AB204169)

Immunohistochemical analysis of human glioma tissue labeling ATRX in the nucleus of tumor cells with ab204169 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody (AB204169)

Immunohistochemical analysis of human cerebral cortex tissue labeling ATRX in the nucleus of neurons and glial cells with ab204169 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody (AB204169)

Immunohistochemical analysis of human uterine cervix tissue labeling ATRX in the nucleus of squamous epithelial cells with ab204169 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-ATRX antibody (AB204169)

Western blot : Anti-ATRX antibody staining at 0.4 ug/ml, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to ATRX. A band was observed at 150/180/350 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in ATRX knockout cell line ab287231. To generate this image, wild-type and ATRX knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 12 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-ATRX antibody (ab204169) at 0.4 µg/mL

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

Western blot - Human ATRX knockout HCT116 cell line (<a href='/ko/products/cell-lines/human-atrx-knockout-hct116-cell-line-ab287231'>ab287231</a>)

Lane 2:

HCT 116 cell lysate at 20 µg

Lane 3:

U-2 OS cell lysate at 20 µg

Secondary

All lanes:

HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 282 kDa

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