AB243036

Anti-AUTS2 antibody [CL7084]

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(3 제품이 사용된 논문 )

Mouse Monoclonal AUTS2 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human AUTS2 aa 1050-1100.

대체 명칭 보기

KIAA0442, AUTS2, Autism susceptibility gene 2 protein

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Paraffin-embedded mouse cerebellum tissue stained for AUTS2 using ab243036 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Paraffin-embedded mouse hippocampus tissue stained for AUTS2 using ab243036 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Paraffin-embedded mouse embryo E14 tissue stained for AUTS2 using ab243036 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Paraffin-embedded mouse embryo E14 tissue stained for AUTS2 using ab243036 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-AUTS2 antibody [CL7084] (AB243036)

All lanes:

Western blot - Anti-AUTS2 antibody [CL7084] (ab243036) at 1 µg/mL

All lanes:

SCLC-21H cell lysate

Predicted band size: 139 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL7084

Isotype

IgG2a

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human AUTS2 aa 1050-1100. The exact immunogen used to generate this antibody is proprietary information.

Q8WXX7

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

AUTS2 also known as the Autism Susceptibility Gene 2 is a protein encoded by the AUTS2 gene and has a mass of approximately 141 kDa. This protein is expressed in several regions of the brain including the cerebral cortex hippocampus and cerebellum. It plays a role in neuronal development where it impacts neural progenitor cell proliferation and differentiation. Expression of AUTS2 is often regulated during key neurodevelopmental stages.

Biological function summary

The AUTS2 protein is involved in chromatin remodeling impacting gene expression regulation during brain development. It functions as part of a complex involving other transcription factors and regulators to influence diverse neural processes. This involvement in chromatin remodeling highlights its role in controlling when and how genes are activated within cells impacting neurodevelopmental processes significantly.

Pathways

AUTS2 participates in the regulation of neuronal signaling pathways such as the Wnt signaling pathway which is key for cell fate determination during development. Proteins like TCF/LEF are related to AUTS2 through the Wnt signaling pathway where they serve as transcriptional mediators affected by the pathway's activity. This connection indicates AUTS2's role in maintaining the balance between progenitor cell proliferation and differentiation in neural tissues.

AUTS2 has a significant association with autism spectrum disorders (ASD) and intellectual disability. Disruptions or mutations in the AUTS2 gene often associate with these neurodevelopmental conditions. The protein also has connections with other proteins like CHD8 which is often mutated in autism suggesting that alterations affecting AUTS2 could influence broader functional networks in the brain responsible for cognitive development and behavior.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 25519132). The PRC1-like complex that contains PCGF5, RNF2, CSNK2B, RYBP and AUTS2 has decreased histone H2A ubiquitination activity, due to the phosphorylation of RNF2 by CSNK2B (PubMed : 25519132). As a consequence, the complex mediates transcriptional activation (PubMed : 25519132). In the cytoplasm, plays a role in axon and dendrite elongation and in neuronal migration during embryonic brain development. Promotes reorganization of the actin cytoskeleton, lamellipodia formation and neurite elongation via its interaction with RAC guanine nucleotide exchange factors, which then leads to the activation of RAC1 (By similarity).

See full target information AUTS2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Acta neuropathologica 150:23 PubMed40906043

2025

TDP-43 dysregulation impairs cholesterol metabolism linked with myelination defects.

Applications

Unspecified application

Species

Unspecified reactive species

Irene García-Toledo,Juan M Godoy-Corchuelo,Luis C Fernández-Beltrán,Zeinab Ali,Ariadna Guindo-Arroyo,Irene Jiménez-Coca,Jesús Jiménez-Rodríguez,Karen Javaloyes-García,Marcos Viñuela,Ulises Gómez-Pinedo,Laura Saiz-Aúz,Alberto Rábano,Estela Área-Gómez,Thomas J Cunningham,Silvia Corrochano

Nature 598:205-213 PubMed34616060

2021

Single-cell epigenomics reveals mechanisms of human cortical development.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan S Ziffra,Chang N Kim,Jayden M Ross,Amy Wilfert,Tychele N Turner,Maximilian Haeussler,Alex M Casella,Pawel F Przytycki,Kathleen C Keough,David Shin,Derek Bogdanoff,Anat Kreimer,Katherine S Pollard,Seth A Ament,Evan E Eichler,Nadav Ahituv,Tomasz J Nowakowski

Nature 578:142-148 PubMed31996853

2020

Cell stress in cortical organoids impairs molecular subtype specification.

Applications

Unspecified application

Species

Unspecified reactive species

Aparna Bhaduri,Madeline G Andrews,Walter Mancia Leon,Diane Jung,David Shin,Denise Allen,Dana Jung,Galina Schmunk,Maximilian Haeussler,Jahan Salma,Alex A Pollen,Tomasz J Nowakowski,Arnold R Kriegstein

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AUTS2 antibody [CL7084]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Western blot - Anti-AUTS2 antibody [CL7084] (AB243036)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise