JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB62490

Anti-Avian Influenza A Hemagglutinin antibody

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Goat Polyclonal Hemagglutinin antibody. Suitable for WB, ELISA and reacts with Recombinant full length protein - HA1(H5N1) samples. Cited in 1 publication.
3 이미지
ELISA - Anti-Avian Influenza A Hemagglutinin antibody (AB62490)
  • ELISA

Supplier Data

ELISA - Anti-Avian Influenza A Hemagglutinin antibody (AB62490)

Validation with H5N1 HA Protein Coating Antigen : H5N1 Hemagglutinin recombinant protein, 2 μg/mL, incubated at 4˚C overnight. Detection Antibodies : ab62490, dilution : 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies : Rabbit anti-goat HRP at 1/10,000 dilution, incubated at RT for 1 hr.

Western blot - Anti-Avian Influenza A Hemagglutinin antibody (AB62490)
  • WB

Supplier Data

Western blot - Anti-Avian Influenza A Hemagglutinin antibody (AB62490)

1h incubation at RT in 5% NFDM/TBST.

Lane 1:

Western blot - Anti-Avian Influenza A Hemagglutinin antibody (ab62490) at 0.5 µg/mL

Lane 2:

Western blot - Anti-Avian Influenza A Hemagglutinin antibody (ab62490) at 1 µg/mL

All lanes:

H5N1 Hemagglutinin recombinant protein at 30 ng

Secondary

All lanes:

Rabbit anti-goat IgG HRP conjugate, at 1/10000 dilution

Predicted band size: 64 kDa

false

Western blot - Anti-Avian Influenza A Hemagglutinin antibody (AB62490)
  • WB

Unknown

Western blot - Anti-Avian Influenza A Hemagglutinin antibody (AB62490)

All lanes:

Western blot - Anti-Avian Influenza A Hemagglutinin antibody (ab62490) at 1 µg/mL

Lane 1:

5ng Recombinant Hemagglutinin

Lane 2:

25ng Recombinant Hemagglutinin

Predicted band size: 64 kDa

Observed band size: 43 kDa

false

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

HA1(H5N1)

Applications

WB, ELISA

applications

Specificity

<p>This antibody reacts with the Hemagglutinin 4 protein from the H5N1 strain of avian influenza A in ELISA and WB. It will detect 10 ng of free peptide at 1 μg/mL.</p>

style
left-column

대체품

Primary Antibodies

AB21297

Anti-Avian Influenza A Hemagglutinin antibody

ELISA - Anti-Avian Influenza A Hemagglutinin antibody (AB21297)

  • 0
  • 0
Primary Antibodies

AB62587

Anti-Avian Influenza A Hemagglutinin antibody

ELISA - Anti-Avian Influenza A Hemagglutinin antibody (AB62587)

  • 0
  • 0

보완 제품

Assay Kits

AB317548

Influenza A Virus Hemagglutinin ELISA Kit

SimpleStep

Sandwich ELISA - Influenza A Virus Hemagglutinin ELISA Kit (AB317548)

  • 0
  • 0
Primary Antibodies

AB205606

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB205606)

  • 5
  • 2
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB9110

Anti-HA tag antibody - ChIP Grade

Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (AB9110)

  • 5
  • 59
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "HA1(H5N1)": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Recombinant full length protein - HA1(H5N1)": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1000 ng/mL", "ELISA-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Avian Influenza A Hemagglutinin (HA) also known as influenza hemagglutinin is a surface glycoprotein with a molecular mass of approximately 63 kilodaltons. HA is expressed on the envelope of the influenza virus where it facilitates viral entry into host cells. This protein binds to sialic acid receptors on the surface of host cells initiating endocytosis and subsequent viral internalization. HA is a critical determinant of host range and antigenicity in avian influenza viruses.
Biological function summary

The avian protein HA plays a central role in the infection cycle of influenza viruses. As part of the viral capsid hemagglutinin undergoes a conformational change upon receptor binding triggering membrane fusion and release of viral RNA into the host cytoplasm. HA is integral to the structure of the virus working alongside neuraminidase which facilitates release of new viral particles. The interaction between hemagglutinin and host receptor is an important step in viral infectivity and immune system evasion.

Pathways

Hemagglutinin interacts closely with cellular and viral pathways influencing viral replication and assembly. HA operates in conjunction with the neuraminidase protein in the viral life cycle particularly in the replication of the genetic material and assembly of the virion. During the entry pathway the low-pH environment in endosomes prompts a change in HA structure leading to fusion of viral and endosomal membranes an important event for viral genome release into the host cytoplasm.

HA of avian influenza is strongly linked to viral pathogenicity and cross-species transmission. Avian influenza A particularly subtypes like H5N1 can cause severe respiratory diseases in birds and humans with HA being an important virulence factor. Mutations in hemagglutinin can alter viral transmissibility and immune escape impacting disease severity. HA also has potential connections with other influenza-related proteins such as matrix protein 2 (M2) which jointly determine viral pathogenicity and host immune responses.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.. Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
See full target information HA

대체 명칭 보기

Hemagglutinin, HA
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 12:623256 PubMed34381441

2021

A20 Establishes Negative Feedback With TRAF6/NF-κB and Attenuates Early Brain Injury After Experimental Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Ji Deng,QuZhen Deji,WangDui Zhaba,Jia-Qiang Liu,Sheng-Qing Gao,Yan-Ling Han,Meng-Liang Zhou,Chun-Xi Wang
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com