AB28824

Anti-Bad (phospho S99) antibody

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(1 리뷰)

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(16 제품이 사용된 논문 )

Rabbit Polyclonal BAD phospho S99 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human BAD pS99 aa 50-150.

대체 명칭 보기

BBC6, BCL2L8, BAD, Bcl2-associated agonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S99) antibody (AB28824)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S99) antibody (AB28824)

Immunohistochemical analysis of paraffin embedded breast carcinoma, using ab28824. Left : Untreated. Right : Treated with synthesized phosphopeptide.

Western blot - Anti-Bad (phospho S99) antibody (AB28824)

WB

Unknown

Western blot - Anti-Bad (phospho S99) antibody (AB28824)

All lanes:

Western blot - Anti-Bad (phospho S99) antibody (ab28824) at 1/500 dilution

Lane 1:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Lane 2:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 18 kDa

Observed band size: 26 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

Immunogen

Synthetic Peptide within Human BAD pS99 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Specificity

Predicited to react with Mouse S136 and Rat S137

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대체품

Primary Antibodies

AB28825

Anti-Bad (phospho S155) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S155) antibody (AB28825)

3
1

Primary Antibodies

AB62465

Anti-Bad antibody

Western blot - Anti-Bad antibody (AB62465)

0
0

Primary Antibodies

AB90435

Anti-Bad antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad antibody (AB90435)

4
1

Primary Antibodies

AB32445

Anti-Bad antibody [Y208]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad antibody [Y208] (AB32445)

4
13

Primary Antibodies

AB220116

Anti-Bad antibody [Y208] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad antibody [Y208] - BSA and Azide free (AB220116)

0
0

Primary Antibodies

AB216829

Anti-Bad (phospho S128) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S128) antibody (AB216829)

0
0

Primary Antibodies

AB129192

Anti-Bad (phospho S112) antibody [EPR1891(2)]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] (AB129192)

0
0

보완 제품

Primary Antibodies

AB32445

Anti-Bad antibody [Y208]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad antibody [Y208] (AB32445)

4
13

Primary Antibodies

AB40776

Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (AB40776)

0
1

Primary Antibodies

AB32158

Anti-Bim antibody [Y36]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody [Y36] (AB32158)

4
6

Primary Antibodies

AB215715

Anti-TGF beta 1 antibody [EPR21143]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

5
7

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Bad protein also known as Bcl-2-associated death promoter plays a critical role in apoptosis regulation. It possesses a molecular weight of approximately 23 kDa. Bad is expressed widely in tissues and is a member of the Bcl-2 family of proteins. This family is well-known for its involvement in the regulation of cell death both promoting and inhibiting depending on the protein’s interactions.

Biological function summary

Its main role is to promote apoptosis by binding to anti-apoptotic proteins like Bcl-2 and Bcl-XL displacing pro-apoptotic Bax and Bak proteins to trigger cell death. Bad functions as part of the mitochondrial apoptosis pathway and is known to form complexes with phosphorylated proteins. Phosphorylation of Bad by kinases can lead to its sequestration to the cytoplasm reducing apoptosis.

Pathways

Its action is pivotal in the PI3K/Akt signaling pathway. This pathway involves growth factors and cell survival signals where phosphorylation of Bad inhibits its pro-apoptotic activity. Bad interacts with proteins like Akt and 14-3-3 to modulate cell survival. Disruption in its regulation through these pathways may lead to abnormal cell survival or death.

Bad has implications in cancer and neurodegenerative diseases. Cancer cells often exhibit impaired apoptotic pathways where overexpression of Bad can ameliorate responsiveness to therapy. In neurodegenerative diseases such as Alzheimer's dysregulation of its function can contribute to cell death. The protein’s interaction with Bcl-2 and Bcl-XL is significant in these contexts influencing disease propagation and severity.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

See full target information BAD pS99

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제품이 사용된 논문 (16)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 42:112595 PubMed37224013

2023

Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Chen,Xuanmao Jiao,Agnese Di Rocco,Duanwen Shen,Shaohua Xu,Adam Ertel,Zuoren Yu,Gabriele Di Sante,Min Wang,Zhiping Li,Timothy G Pestell,Mathew C Casimiro,Emmanuel Skordalakes,Samuel Achilefu,Richard G Pestell

Physiological reports 10:e15241 PubMed35388988

2022

FGF7 peptide (FGF7p) mimetic mitigates bladder urothelial injury from cyclophosphamide.

Applications

Unspecified application

Species

Unspecified reactive species

Sridhar Tatarao Narla,Lori Rice,David Ostrov,Steven G Swarts,Dietmar W Siemann,Daniel Scott Bushnell,Jacqueline G Holden,Joanne Lindsey Duara,Carlton Matthew Bates

The American journal of pathology 192:604-612 PubMed35063403

2022

AKT Signaling Downstream of KGF Is Necessary and Sufficient for Blocking Cyclophosphamide Bladder Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sridhar T Narla,Daniel S Bushnell,Joanne L Duara,Carlton M Bates

Frontiers in pharmacology 12:713715 PubMed34381366

2021

The Dual Dose-Dependent Effects of Corticosterone on Hippocampal Cell Apoptosis After Traumatic Brain Injury Depend on the Activation Ratio of Mineralocorticoid Receptors to Glucocorticoid Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zhang,Mengshi Yang,Qiongyu Yan,Xiaojian Xu,Fei Niu,Jinqian Dong,Yuan Zhuang,Shenghua Lu,Qianqian Ge,Baiyun Liu

Journal of neuroinflammation 17:318 PubMed33100225

2020

Corticosteroid receptor rebalancing alleviates critical illness-related corticosteroid insufficiency after traumatic brain injury by promoting paraventricular nuclear cell survival via Akt/CREB/BDNF signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zhang,Miao Bai,Xiaojian Xu,Mengshi Yang,Fei Niu,Fei Gao,Baiyun Liu

Cell reports 32:108151 PubMed32937140

2020

Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Chen,Xuanmao Jiao,Agnese Di Rocco,Duanwen Shen,Shaohua Xu,Adam Ertel,Zuoren Yu,Gabriele Di Sante,Min Wang,Zhiping Li,Timothy G Pestell,Mathew C Casimiro,Emmanuel Skordalakes,Samuel Achilefu,Richard G Pestell

BioMed research international 2019:7298539 PubMed31772936

2019

Dihydrochalcone Derivative Induces Breast Cancer Cell Apoptosis via Intrinsic, Extrinsic, and ER Stress Pathways but Abolishes EGFR/MAPK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wasitta Rachakhom,Patompong Khaw-On,Wilart Pompimon,Ratana Banjerdpongchai

PloS one 13:e0196805 PubMed29723246

2018

Early changes in rpS6 phosphorylation and BH3 profiling predict response to chemotherapy in AML cells.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Grundy,Thomas Jones,Liban Elmi,Michael Hall,Adam Graham,Nigel Russell,Monica Pallis

Cellular physiology and biochemistry : internation 41:451-465 PubMed28214890

2017

A New Perspective for Osteosarcoma Therapy: Proteasome Inhibition by MLN9708/2238 Successfully Induces Apoptosis and Cell Cycle Arrest and Attenuates the Invasion Ability of Osteosarcoma Cells in Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Renhao Liu,Chunjiang Fu,Jiabing Sun,Xvming Wang,Shuo Geng,Xiaoyu Wang,Jilong Zou,Zhenggang Bi,Chenglin Yang

Oncotarget 7:68314-68327 PubMed27582542

2016

Propranolol induced G0/G1/S phase arrest and apoptosis in melanoma cells via AKT/MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chengfang Zhou,Xiang Chen,Weiqi Zeng,Cong Peng,Gang Huang,Xian'an Li,Zhengxiao Ouyang,Yi Luo,Xuezheng Xu,Biaobo Xu,Weili Wang,Ruohui He,Xu Zhang,Liyang Zhang,Jie Liu,Todd C Knepper,Yijing He,Howard L McLeod

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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