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AB244692

Anti-Bag3 antibody [EPR20207-4] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal BAG3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-Bag3 antibody [EPR20207-4] - BSA and Azide free (Capture) (AB244692)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Bag3 antibody [EPR20207-4] - BSA and Azide free (Capture) (AB244692)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab227900).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20207-4

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB227900

Human BAG3 ELISA Kit

Recombinant|SimpleStep

ELISA - Human BAG3 ELISA Kit (AB227900)

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Sample Prep & Detection Kits

AB201806

DyLight® 800 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (AB201806)

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Sample Prep & Detection Kits

AB154876

Gold Conjugation Kit (80nm, 20 OD)

Conjugation - Gold Conjugation Kit (80nm, 20 OD) (AB154876)

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Primary Antibodies

AB259551

Anti-LRG1/LRG antibody [EPR22432-122] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-LRG1/LRG antibody [EPR22432-122] - BSA and Azide free (Capture) (AB259551)

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Primary Antibodies

AB245087

Anti-Vaspin antibody [EPR21830-62] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Vaspin antibody [EPR21830-62] - BSA and Azide free (Detector) (AB245087)

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Primary Antibodies

AB245098

Anti-IGFBP6 antibody [EPR21809-135] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IGFBP6 antibody [EPR21809-135] - BSA and Azide free (Detector) (AB245098)

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Primary Antibodies

AB252631

Anti-Serum Amyloid P/SAP antibody [EPR20042-239] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Serum Amyloid P/SAP antibody [EPR20042-239] - BSA and Azide free (Detector) (AB252631)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab244692 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human BAG3. The recommended pair for sandwich ELISA is:
Capture: ab244692, Human BAG3 Capture Antibody (unconjugated)
Detector: ab244958, Human BAG3 Detector Antibody (unconjugated)
The reference range value is 39.1 - 2500 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Bcl-2-associated athanogene 3 commonly known as Bag3 is a protein with a molecular weight of approximately 74 kDa. It plays an important role in apoptosis regulation and stress responses by interacting with heat shock proteins. Bag3 is expressed in various tissues including the heart skeletal muscles and certain types of neurons. It functions mechanically by binding to chaperones like Hsp70 modulating their activity and helping in protein folding and degradation processes.
Biological function summary

Bag3 contributes to cell survival by assisting in protein homeostasis and mitigating stress-related cellular damage. It acts as a co-chaperone and forms part of complexes with Hsp70 HspB8 and CHIP among others. These interactions enhance the cellular capacity to handle misfolded proteins preventing the aggregation that could lead to cell toxicity. In muscle cells Bag3 plays a role in maintaining structural integrity by participating in the assembly of myofibrils critical for muscle function.

Pathways

The protein is involved in autophagy and apoptosis regulation. It actively participates in the ubiquitin-proteasome system and chaperone-mediated autophagy collaborating closely with proteins like HspB8 and Hsp70. The integration of Bag3 within these pathways highlights its involvement in removing damaged proteins and preserving cellular function during stress conditions. Additionally Bag3 contributes to the regulation of the NF-kB pathway which is involved in the immune response and inflammation.

Mutations or dysregulation of Bag3 associate with conditions such as myopathy and certain cancers. In myopathy aberrations in Bag3 affect muscle resilience and repair leading to structural muscle defects. In cancer Bag3's role in inhibiting apoptosis promotes tumor cell survival making it a target for therapeutic interventions. The protein has connections with p53 in cancer contexts where it influences cell death and survival decisions. Understanding Bag3's interactions and functions in these diseases opens new opportunities for developing treatments targeting these pathways.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Co-chaperone and adapter protein that connects different classes of molecular chaperones including heat shock proteins 70 (HSP70s), e.g. HSPA1A/HSP70 or HSPA8/HSC70, and small heat shock proteins (sHSPs), e.g. HSPB8 (PubMed : 27884606, PubMed : 30559338). Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from HSP70s, thereby triggering client protein release (PubMed : 27884606, PubMed : 30559338). Nucleotide release is mediated via BAG3 binding to the nucleotide-binding domain (NBD) of HSP70s, whereas client release is mediated via binding to the substrate-binding domain (SBD) (PubMed : 27474739, PubMed : 9873016). Has anti-apoptotic activity (PubMed : 10597216). Plays a role in the HSF1 nucleocytoplasmic transport (PubMed : 26159920).
See full target information BAG3

대체 명칭 보기

BIS, BAG3, BAG family molecular chaperone regulator 3, BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, Docking protein CAIR-1
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Product promise

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