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AB252258

Anti-Bax antibody [5B7] - BSA and Azide free

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Mouse Recombinant Monoclonal BAX antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse samples.

대체 명칭 보기

Apoptosis regulator BAX, Bax

3 이미지
Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3191).

Intracellular Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling Bax with ab3191 at 1.043μg/ml (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution.

Immunoprecipitation - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)
  • IP

Supplier Data

Immunoprecipitation - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3191).

Bax was immunoprecipitated from RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab190908 at 13.9μg/ml . Western blot was performed from the immunoprecipitate using ab3191 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used as secondary antibody at 1/50000 dilution.
Lane 1 : RAW 264.7 whole cell lysate 10ug (Input).
Lane 2 : ab3191 IP in RAW 264.7 whole cell lysate.
Lane 3 : Mouse monoclonal IgG instead of ab3191 in RAW 264.7 whole cell lysate.
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Bax antibody [5B7] (<a href='/ko/products/primary-antibodies/bax-antibody-5b7-ab3191'>ab3191</a>)

Predicted band size: 21 kDa

false

Western blot - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)
  • WB

Supplier Data

Western blot - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3191).

Blocking/Dilution buffer : 5% NFDM/TBST.

Lane 1:

Western blot - Anti-Bax antibody [5B7] (<a href='/ko/products/primary-antibodies/bax-antibody-5b7-ab3191'>ab3191</a>) at 0.417 mg/mL

Lane 2:

Western blot - Anti-Bax antibody [5B7] (<a href='/ko/products/primary-antibodies/bax-antibody-5b7-ab3191'>ab3191</a>) at 0.417 µg/mL

Lane 1:

A20 (Mouse reticulum sarcoma B lymphocyte) lysate at 20 µg

Lane 2:

C2C12 (Mouse myoblasts) lysate at 20 µg

Predicted band size: 21 kDa

false

Exposure time: 26s

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

5B7

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Antibody raised against a synthetic peptide that is not shared by rat or human Bax.

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대체품

Primary Antibodies

AB262929

Anti-Bax antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody (AB262929)

  • 0
  • 0
Primary Antibodies

AB81083

Anti-Bax antibody [SP47]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

  • 0
  • 0
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
Primary Antibodies

AB216985

Anti-Bax antibody [E63] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] - BSA and Azide free (AB216985)

  • 0
  • 0
Primary Antibodies

AB240930

Anti-Bax antibody [SP47] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

  • 0
  • 0
Primary Antibodies

AB182733

Anti-Bax antibody [EPR18283]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunoprecipitation - Anti-Bax antibody [EPR18283] (AB182733)

  • 4
  • 6

보완 제품

Assay Kits

AB199080

Human Bax ELISA Kit

Recombinant|SimpleStep

ELISA - Human Bax ELISA Kit (AB199080)

  • 0
  • 0
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13
Primary Antibodies

AB184787

Anti-Caspase-3 antibody [EPR18297]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [EPR18297] (AB184787)

  • 3
  • 3
Primary Antibodies

AB202068

Anti-Caspase-9 antibody [EPR18107]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [EPR18107] (AB202068)

  • 5
  • 4
Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "13.9 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.417 µg/mL", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1.043 µg/mL", "FlowCytIntra-species-notes": "<p></p>" } } }
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제품 세부 정보

ab252258 is the carrier-free version of ab3191.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.
Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production.
See full target information Bax
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com