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AB240930

Anti-Bax antibody [SP47] - BSA and Azide free

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(1 출판물)

Rabbit Recombinant Monoclonal BAX antibody. Carrier free. Suitable for IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.
12 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

Formalin-fixed, paraffin-embedded human cervix tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

Formalin-fixed, paraffin-embedded human breast tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

Formalin-fixed, paraffin-embedded human skin tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

Formalin-fixed, paraffin-embedded human esophagus tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

Formalin-fixed, paraffin-embedded human breast tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Bax with ab81083 at 1/100 dilution (2.42 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human skin.

This image was generated using ab81083, the same clone, but with a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling Bax with ab81083 at 1/100 dilution (2.42 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on mouse skin.

This image was generated using ab81083, the same clone, but with a different buffer formulation.

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP47

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB111391

Anti-Bax (phospho S184) antibody

  • 0
  • 0
Primary Antibodies

AB262929

Anti-Bax antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody (AB262929)

  • 0
  • 0
Primary Antibodies

AB192819

Anti-Bax (phospho T167) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Bax (phospho T167) antibody (AB192819)

  • 0
  • 0
Primary Antibodies

AB216494

Anti-Bax antibody

Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody (AB216494)

  • 0
  • 0
Primary Antibodies

AB104156

Anti-Bax antibody

KO Validated

Western blot - Anti-Bax antibody (AB104156)

  • 0
  • 0
Primary Antibodies

AB212790

Anti-Bax antibody [2D2] - BSA and Azide free

Recombinant

Immunoprecipitation - Anti-Bax antibody [2D2] - BSA and Azide free (AB212790)

  • 0
  • 0
Primary Antibodies

AB270742

Anti-Bax antibody [2D2]

Recombinant|Trial Size

Immunoprecipitation - Anti-Bax antibody [2D2] (AB270742)

  • 0
  • 0
Primary Antibodies

AB205822

HRP Anti-Bax antibody [E63]

RabMAb|Recombinant|KO Validated

Western blot - HRP Anti-Bax antibody [E63] (AB205822)

  • 0
  • 0
Primary Antibodies

AB182734

Anti-Bax antibody [EPR18284]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunoprecipitation - Anti-Bax antibody [EPR18284] (AB182734)

  • 0
  • 0
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
Primary Antibodies

AB232479

Anti-Bax antibody [EPR18283] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-Bax antibody [EPR18283] - BSA and Azide free (AB232479)

  • 0
  • 0
Primary Antibodies

AB216985

Anti-Bax antibody [E63] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] - BSA and Azide free (AB216985)

  • 0
  • 0
Primary Antibodies

AB53154

Anti-Bax antibody

Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody (AB53154)

  • 1
  • 1
Primary Antibodies

AB263897

Anti-Bax antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody (AB263897)

  • 0
  • 0
Primary Antibodies

AB252258

Anti-Bax antibody [5B7] - BSA and Azide free

Recombinant

Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)

  • 0
  • 0

보완 제품

Assay Kits

AB199080

Human Bax ELISA Kit

Recombinant|SimpleStep

ELISA - Human Bax ELISA Kit (AB199080)

  • 0
  • 0
Sample Prep & Detection Kits

AB102921

Streptavidin Conjugation Kit - Lightning-Link®

Conjugation - Streptavidin Conjugation Kit - Lightning-Link® (AB102921)

  • 0
  • 0
Sample Prep & Detection Kits

AB201808

Gold Conjugation Kit (10 nm, 20 OD)

Conjugation - Gold Conjugation Kit (10 nm, 20 OD) (AB201808)

  • 0
  • 0
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13
Primary Antibodies

AB184787

Anti-Caspase-3 antibody [EPR18297]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [EPR18297] (AB184787)

  • 3
  • 3
Primary Antibodies

AB32370

Anti-Bcl-XL antibody [E18]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [E18] (AB32370)

  • 5
  • 10
Primary Antibodies

AB2013

Anti-Caspase-9 antibody

Western blot - Anti-Caspase-9 antibody (AB2013)

  • 4
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Primary antibody incubation for 30 minutes at room temperature.</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Primary antibody incubation for 30 minutes at room temperature.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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제품 세부 정보

ab240930 is the carrier-free version of ab81083.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
Purification 관련 사항
Purified from TCS by protein A/G.
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.
Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Plays a role in the mitochondrial apoptotic process (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 36361894, PubMed : 8358790, PubMed : 8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed : 21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816).
See full target information BAX

대체 명칭 보기

BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 21:486 PubMed33968202

2021

Circular RNA UBAP2 promotes the proliferation of prostate cancer cells via the miR-1244/MAP3K2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Li,Baihetiya Azhati,Wenguang Wang,Mulati Rexiati,Chen Xing,Yujie Wang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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