AB275961

Anti-BCKDHA (phospho S292) antibody [BLR060F]

20ul selling size
Recombinant
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Rabbit Recombinant Monoclonal BCKDHA phospho S292 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BCKDHA phospho S292.

대체 명칭 보기

Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, BCKDE1A, BCKDH E1-alpha, BCKDHA

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Supplier Data

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

Chemiluminescence with an exposure time of 30 seconds.

Lower Panel : Rabbit anti-RPL10

All lanes:

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 5:

LNCaP (human prostate cancer cell line) whole cell lysate

Lane 6:

GaMG whole cell lysate

Lane 7:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 8:

MCF-7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 9:

Differentiated 3T3-L1 whole cell lysate

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

All lanes:

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution

Lane 1:

HEK293T cells mock treated (-) whole cell lysate at 50 µg

Lane 2:

HEK293T cells treated with phosphatases (+) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 50 kDa

Observed band size: 49 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

All lanes:

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution

Lane 1:

NIH 3T3 whole cell lysate at 50 µg

Lane 2:

CT26 whole cell lysate at 50 µg

Lane 3:

TCMK-1 whole cell lysate at 50 µg

Lane 4:

BW5147.3 whole cell lysate at 50 µg

Lane 5:

CH27 whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 50 kDa

Observed band size: 49 kDa

true

Exposure time: 75s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR060F

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human BCKDHA phospho S292. The exact immunogen used to generate this antibody is proprietary information.

P12694

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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특성 및 보관 정보

제형

Liquid

Purification 관련 사항

Recombinant antibody was purified from cell culture supernatant.

보관 버퍼

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99.81% Borate buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.

See full target information BCKDHA phospho S292

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental physiology 108:961-974 PubMed37139700

2023

High doses of rosuvastatin induce impaired branched-chain amino acid catabolism and lead to insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Bai,Xingzhen Long,Fang Song,Baolin Chen,Changcheng Sheng,Cailin Tang,Li Li,Jiaxing Zhang,Rui Zhang,Jiquan Zhang,Jiali Li

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BCKDHA (phospho S292) antibody [BLR060F]

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise