AB16904

Anti-Bcl-2 antibody [10C4]

Recombinant
각 태그의 뜻

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(8 제품이 사용된 논문 )

Mouse Recombinant Monoclonal Bcl-2 antibody. Suitable for WB and reacts with Mouse samples. Cited in 8 publications.

대체 명칭 보기

Bcl-2, Bcl2, Apoptosis regulator Bcl-2

1 이미지

Lab

Western blot - Anti-Bcl-2 antibody [10C4] (AB16904)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 15 secs; Lane 2,3 : 180 secs.

All lanes:

Western blot - Anti-Bcl-2 antibody [10C4] (ab16904) at 0.488 µg/mL

Lane 1:

Mouse thymus tissue lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 26 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

10C4

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.488 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.

Biological function summary

The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.

Pathways

Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.

The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy : interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (By similarity). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).

See full target information Bcl2

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제품이 사용된 논문 (8)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nutrients 17: PubMed40077694

2025

Unraveling the Osteogenic Activity and Molecular Mechanism of an Antioxidant Collagen Peptide in MC3T3-E1 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yali Wang,Yue Wang,Xiaoyan Zhuang,Yonghui Zhang,Baishan Fang,Yousi Fu

Physiological research 73:755-767 PubMed39545790

2024

MiR-92a-3p Promotes Renal Injury and Fibrosis Through Facilitating M1 Macrophage Polarization via Targeting LIN28A.

Applications

Unspecified application

Species

Unspecified reactive species

M Xu,X Zeng,M Pan,R Chen,Y Bai,J He,C Wang,Y Qi,Q Sun,C Wang,N An

Neuroscience bulletin 40:35-49 PubMed37608137

2023

Impairment of Autophagic Flux After Hypobaric Hypoxia Potentiates Oxidative Stress and Cognitive Function Disturbances in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhui Dai,Yuan Feng,Chuanhao Lu,Hongchen Zhang,Wenke Ma,Wenyu Xie,Xiuquan Wu,Peng Luo,Lei Zhang,Fei Fei,Zhou Fei,Xia Li

Frontiers in surgery 10:1073557 PubMed36860953

2023

Oscillatory shear stress promotes vein graft intimal hyperplasia NADPH oxidase-related pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqing Yao,Huanhuan Li,Xiangyi Zuo,Chunkai Wang,Yelei Xiao,Yu Zhao,Xuehu Wang

Bioengineered 13:14426-14437 PubMed36694458

2023

Long non-coding RNA lincRNA-erythroid prosurvival attenuates inflammation by enhancing myosin heavy chain 6 stability through recruitment of heterogeneous nuclear ribonucleoprotein L in myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Huizhen Zhang,Xuejun Kou,Dong Xiao,Zhangjian Yu

Experimental and therapeutic medicine 23:350 PubMed35493436

2022

CD47 antibody protects mice from doxorubicin-induced myocardial damage by suppressing cardiomyocyte apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Hao,Lianghua Chen,Zhilong Jiang

Frontiers in oncology 11:633210 PubMed33854970

2021

Glycolysis and Fatty Acid Oxidation Inhibition Improves Survival in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly J McKelvey,Erica B Wilson,Susan Short,Alan A Melcher,Michael Biggs,Connie I Diakos,Viive M Howell

Environmental toxicology and pharmacology 31:262-5 PubMed21359097

2011

Isoflurane preconditioning reduces oxygen-glucose deprivation-induced neuronal injury via B-cell lymphoma 2 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Sook Gwak,Lin Cao,Liaoliao Li,Zhiyi Zuo

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bcl-2 antibody [10C4]

Western blot - Anti-Bcl-2 antibody [10C4] (AB16904)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (8)

Product promise