AB73985

Anti-Bcl-2 (phospho S87) antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal Bcl-2 phospho S87 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human BCL2 pS87.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 (phospho S87) antibody (AB73985)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 (phospho S87) antibody (AB73985)

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using ab73985 at 1/50 dilution in the presence (right panel) or absence (left panel) of immunising phosphopeptide.

Western blot - Anti-Bcl-2 (phospho S87) antibody (AB73985)

WB

Unknown

Western blot - Anti-Bcl-2 (phospho S87) antibody (AB73985)

All lanes:

Western blot - Anti-Bcl-2 (phospho S87) antibody (ab73985) at 1/500 dilution

Lane 1:

Extracts from HeLa cells, treated with nocodazole (1ug/ml, 18hours) at 5 µg

Lane 2:

Extracts from HeLa cells, treated with nocodazole (1ug/ml, 18hours) at 5 µg with immunising phosphopeptide

Predicted band size: 26 kDa

Observed band size: 28 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

Immunogen

Synthetic Peptide within Human BCL2 pS87. The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

5
13

Primary Antibodies

AB233694

Anti-Bcl-2 (phospho S70) antibody [EPR21162] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Bcl-2 (phospho S70) antibody [EPR21162] - BSA and Azide free (AB233694)

0
0

Primary Antibodies

AB185002

Anti-Bcl-2 antibody [E17] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (AB185002)

0
0

Primary Antibodies

AB219608

Anti-Bcl-2 antibody [EPR17509] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [EPR17509] - BSA and Azide free (AB219608)

0
0

Primary Antibodies

AB32124

Anti-Bcl-2 antibody [E17]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] (AB32124)

3
14

Primary Antibodies

AB218123

Anti-Bcl-2 (phospho S70) antibody [EPR21162]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (AB218123)

0
0

보완 제품

Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

5
13

Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

4
12

Primary Antibodies

AB32042

Anti-Cleaved Caspase-3 antibody [E83-77]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-Cleaved Caspase-3 antibody [E83-77] (AB32042)

4
28

Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

5
133

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

ab73985 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.

Biological function summary

The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.

Pathways

Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.

The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed : 1508712, PubMed : 8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed : 11368354). Appears to function in a feedback loop system with caspases (PubMed : 11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed : 11368354). Also acts as an inhibitor of autophagy : interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed : 18570871, PubMed : 20889974, PubMed : 21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).

See full target information BCL2 pS87

대체 명칭 보기

Apoptosis regulator Bcl-2, BCL2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Environmental toxicology 38:1678-1689 PubMed37087754

2023

NEDD4L inhibits migration, invasion, cisplatin resistance and promotes apoptosis of bladder cancer cells by inactivating the p62/Keap1/Nrf2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wu,Huijiang Zhang,Shengjie You,Zhaoyu Xu,Xiang Liu,Xuedong Chen,Weili Zhang,Junjie Ye,Peng Li,Xiaoqing Zhou

Iranian journal of pharmaceutical research : IJPR 11:1285-90 PubMed24250563

2013

BCL-2 and Bax Expression in Skin Flaps Treated with Finasteride or Azelaic Acid.

Applications

Unspecified application

Species

Unspecified reactive species

Seyyed Abdulmajid Ayatollahi,Marjan Ajami,Hamed Reyhanfard,Yasin Asadi,Mansour Nassiri-Kashani,Mehdi Rashighi Firoozabadi,Sayed Hossein Davoodi,Esmaeil Habibi,Hamidreza Pazoki-Toroudi

The Journal of physiology 589:3349-69 PubMed21540338

2011

Protective effect of caspase inhibition on compression-induced muscle damage.

Applications

WB

Species

Rat

Bee T Teng,Eric W Tam,Iris F Benzie,Parco M Siu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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