AB236221

Anti-Bcl2 alpha antibody [SP66] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Bcl-2 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications.

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2 alpha antibody [SP66] - BSA and Azide free (AB236221)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2 alpha antibody [SP66] - BSA and Azide free (AB236221)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Bcl2 alpha with ab93884 at 1/100 dilution in immunohistochemical analysis. DAB staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab93884).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP66

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Boil tissue section in citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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제품 세부 정보

ab236221 is the carrier-free version of ab93884.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from TCS by protein A/G.

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Bcl2 alpha also known as B-cell lymphoma 2 is a protein with a molecular mass of approximately 26 kDa. This protein plays an important role mechanically as a regulator of apoptosis actively inhibiting cell death in various cell types. Expressed in cells of hematopoietic origin Bcl2 alpha localizes mainly within the outer mitochondrial membrane nuclear envelope and endoplasmic reticulum. The protein's prominent function contributes to balancing cell survival and death making it significant in cellular homeostasis.

Biological function summary

Bcl2 alpha controls the intrinsic pathway of apoptosis. It is a part of the Bcl-2 family which consists of proteins that either promote or inhibit apoptosis. Bcl2 alpha interacts with other Bcl-2 family members forming either pro-apoptotic or anti-apoptotic complexes. It ensures cell survival by preventing the release of cytochrome c from mitochondria and interacting with pro-apoptotic proteins like Bax and Bak. This interaction obstructs the activation of caspase enzymes which are critical in the execution of apoptosis.

Pathways

Bcl2 alpha functions within the apoptotic and mitochondrial pathways. In the apoptotic pathway Bcl2 alpha plays an anti-apoptotic role by modulating the activity of pro-apoptotic proteins mainly through its interactions with Bax and Bak. Within the mitochondrial pathway Bcl2 alpha affects the permeability of the mitochondrial membrane thereby influencing the release of factors that initiate caspase activation. These pathways involve interconnected roles with other key proteins these roles affecting processes such as cell cycle regulation and response to cellular stress.

Bcl2 alpha is notably associated with certain cancers and neurodegenerative diseases. In cancers like lymphoma Bcl2 alpha is often overexpressed which leads to increased cell survival and resistance to apoptosis therefore contributing to tumor progression. The protein is also linked to neurodegenerative diseases where it may influence neuronal survival. Connections with proteins like myc in cancers and others in neurological contexts further highlight the importance of Bcl2 alpha in these disorders. Understanding its role offers potential therapeutic targets for treatment strategies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed : 1508712, PubMed : 8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed : 11368354). Appears to function in a feedback loop system with caspases (PubMed : 11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed : 11368354). Also acts as an inhibitor of autophagy : interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed : 18570871, PubMed : 20889974, PubMed : 21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).

See full target information BCL2

대체 명칭 보기

Apoptosis regulator Bcl-2, BCL2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer cell 42:444-463.e10 PubMed38428410

2024

Multi-omic profiling of follicular lymphoma reveals changes in tissue architecture and enhanced stromal remodeling in high-risk patients.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea J Radtke,Ekaterina Postovalova,Arina Varlamova,Alexander Bagaev,Maria Sorokina,Olga Kudryashova,Mark Meerson,Margarita Polyakova,Ilia Galkin,Viktor Svekolkin,Sergey Isaev,Daniil Wiebe,Anna Sharun,Alexander Sarachakov,Grigory Perelman,Yaroslav Lozinsky,Ziv Yaniv,Bradley C Lowekamp,Emily Speranza,Li Yao,Stefania Pittaluga,Arthur L Shaffer,Danny Jonigk,James D Phelan,Theresa Davies-Hill,Da Wei Huang,Pavel Ovcharov,Krystle Nomie,Ekaterina Nuzhdina,Nikita Kotlov,Ravshan Ataullakhanov,Nathan Fowler,Michael Kelly,Jagan Muppidi,Jeremy L Davis,Jonathan M Hernandez,Wyndham H Wilson,Elaine S Jaffe,Louis M Staudt,Mark Roschewski,Ronald N Germain

Cancer medicine 13:e6851 PubMed38148602

2023

Improved outcomes of localized diffuse large B-cell lymphoma at the Waldeyer ring in comparison to the sinonasal area in the rituximab era.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Li Ma,Ruey-Long Hong,Pei-Jen Lou,Ming Yao,Shang-Ju Wu,Chung-Wu Lin,Chun-Wei Wang,Chin-Hao Chang,Ann-Lii Cheng,Sung-Hsin Kuo

Nature communications 11:1711 PubMed32249764

2020

Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer M Rosenbluth,Ron C J Schackmann,G Kenneth Gray,Laura M Selfors,Carman Man-Chung Li,Mackenzie Boedicker,Hendrik J Kuiken,Andrea Richardson,Jane Brock,Judy Garber,Deborah Dillon,Norman Sachs,Hans Clevers,Joan S Brugge

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bcl2 alpha antibody [SP66] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2 alpha antibody [SP66] - BSA and Azide free (AB236221)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise