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AB240925

Anti-Bcl6 antibody [EP529Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal BCL6 antibody. Carrier free. Suitable for WB, IP and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

BCL5, LAZ3, ZBTB27, ZNF51, BCL6, B-cell lymphoma 6 protein, BCL-6, B-cell lymphoma 5 protein, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51, BCL-5

2 이미지
Immunoprecipitation - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (AB240925)
  • IP

Lab

Immunoprecipitation - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (AB240925)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab33901). Bcl6 was immunoprecipitated from Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate with ab33901 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab33901 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution. Lane 1 (Input) : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate, 10 μg Lane 2 (+) : Ramos whole cell lysate Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab33901 in Ramos whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Bcl6 antibody [EP529Y] (<a href='/ko/products/primary-antibodies/bcl6-antibody-ep529y-ab33901'>ab33901</a>) at 1/1000 dilution

Lane 1:

Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 10 µg

Lane 2:

Ramos whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 79 kDa

Observed band size: 78 kDa

false

Western blot - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (AB240925)
  • WB

Supplier Data

Western blot - Anti-Bcl6 antibody [EP529Y] - BSA and Azide free (AB240925)

Blocking and diluting buffer : 5% NFDM/TBST.

This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol section of the website and/or here.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab33901).

All lanes:

Western blot - Anti-Bcl6 antibody [EP529Y] (<a href='/ko/products/primary-antibodies/bcl6-antibody-ep529y-ab33901'>ab33901</a>) at 1/1000 dilution

Lane 1:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method at 15 µg

Lane 2:

Ramos (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 79 kDa

false

Exposure time: 10s

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP529Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB218951

Anti-Bcl6 antibody [BCL6/1526] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [BCL6/1526] - BSA and Azide free (AB218951)

  • 0
  • 0
Primary Antibodies

AB218952

Anti-Bcl6 antibody [BCL6/1527] - BSA and Azide free

Protein Array - Anti-Bcl6 antibody [BCL6/1527] - BSA and Azide free (AB218952)

  • 0
  • 0
Primary Antibodies

AB218509

Anti-Bcl6 antibody [BCL6/1527]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [BCL6/1527] (AB218509)

  • 4
  • 1
Primary Antibodies

AB203619

Anti-Bcl6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody (AB203619)

  • 0
  • 0
Primary Antibodies

AB19011

Anti-Bcl6 antibody

Western blot - Anti-Bcl6 antibody (AB19011)

  • 0
  • 0
Primary Antibodies

AB183308

Anti-Bcl6 antibody [SP155] - C-terminal

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - C-terminal (AB183308)

  • 2
  • 2
Primary Antibodies

AB243920

Anti-Bcl6 antibody [SP155] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (AB243920)

  • 0
  • 0
Primary Antibodies

AB218507

Anti-Bcl6 antibody [BCL6/1475]

Western blot - Anti-Bcl6 antibody [BCL6/1475] (AB218507)

  • 0
  • 0
Primary Antibodies

AB220092

Anti-Bcl6 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Bcl6 antibody (AB220092)

  • 0
  • 0

보완 제품

Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }
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제품 세부 정보

ab240925 is the carrier-free version of ab33901.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Bcl6 protein also known as B-cell lymphoma 6 is a transcriptional repressor important for the germinal center formation in B-cells. It has a molecular weight of approximately 95 kDa. Bcl6 is expressed mainly in B-cells T-cells and certain subsets of dendritic cells. It functions by binding to target DNA sequences where it represses the expression of specific genes leading to controlled cell proliferation differentiation and apoptosis. Researchers often study Bcl6 through the use of monoclonal antibodies particularly in immunohistochemistry (IHC).
Biological function summary

Bcl6 plays essential roles in immune response regulation and lymphocyte development. In germinal centers Bcl6 influences the process of somatic hypermutation and class switch recombination in B-cells which is a part of generating antibody diversity. It operates as a central player in a multiprotein complex that includes corepressors such as SMRT and NCoR which facilitate the repression of its target genes. This regulatory action helps maintain the precise balance of immune cell functions.

Pathways

Bcl6 integrates into the B-cell receptor (BCR) signaling and the JAK-STAT pathway. It interacts with molecules like STAT1 and STAT3 positively influencing the survival and proliferation of B-cells. In these pathways Bcl6's activity modulates the transcriptional landscape that determines cell fate decisions affecting cellular processes such as proliferation and survival in response to external cues.

Bcl6 is relevant to B cell lymphomas specifically diffuse large B-cell lymphoma (DLBCL) where it is often overexpressed contributing to malignancy by preventing cell death. This pathology frequently involves chromosomal translocations that juxtapose the Bcl6 gene with immunoglobulin genes. The relationship between Bcl6 and other proteins like Bcl2 is noteworthy in this context as Bcl2 inhibits apoptosis further contributing to cancer progression. Mutations and dysregulated expression of Bcl6 have also shown links to autoimmune diseases where its role in cell differentiation and survival may lead to aberrant immune responses.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunoglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.
See full target information BCL6
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cellular and molecular medicine 29:e70693 PubMed40703032

2025

A PEDF-Derived Short Peptide Prevents Sodium Iodate-Induced Retinal Degeneration in Rats by Activating the SLC7A11/GSH/GPX4 Pathway in the RPE Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tsung-Chuan Ho,Shawn-H Tsai,Shu-I Yeh,Ming-Hui Sun,Yeou-Ping Tsao
제품이 사용된 논문 모두 보기
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Product promise

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