Anti-BCL9L antibody

• ICC

Unknown

Immunocytochemistry - Anti-BCL9L antibody (AB113110)

Immunocytochemistry of Bcl9L in HeLa cells with ab113110 at 10 ug/mL.

• WB

Lab

Western blot - Anti-BCL9L antibody (AB113110)

Western blot : Anti-BCL9L antibody (ab113110) staining at 1 ug/ml, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab113110 was shown to bind specifically to BCL9L. A band was observed at 190 kDa in wild-type A549 cell lysates with no signal observed at this size in BCL9L knockout cell line. To generate this image, wild-type and BCL9L knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-BCL9L antibody (ab113110) at 1 µg/mL

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human BCL9L Knockout A549 cell line (<a href='/ko/products/cell-lines/human-bcl9l-knockout-a549-cell-line-ab324209'>ab324209</a>) at 30 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 190 kDa

false

• WB

Unknown

Western blot - Anti-BCL9L antibody (AB113110)

All lanes:

Western blot - Anti-BCL9L antibody (ab113110) at 1 µg/mL

Lane 1:

HeLa cell lysate

Lane 2:

HeLa cell lysate with Blocking peptide

Predicted band size: 157 kDa

false