AB222406

Anti-Bcr antibody [EPR22062]

RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal BCR antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

대체 명칭 보기

BCR1, D22S11, BCR, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26

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Flow Cytometry (Intracellular) - Anti-Bcr antibody [EPR22062] (AB222406)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Bcr antibody [EPR22062] (AB222406)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling Bcr with ab222406 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-Bcr antibody [EPR22062] (AB222406)

Bcr was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab222406 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222406 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection antibody at 1/1000 dilution.

Lane 1 : HeLa whole cell lysate 10μg (Input).
Lane 2 : ab222406 IP in HeLa whole cell lysate (+)
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab222406 in HeLa whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds.

The 160 kDa and 130 kDa bands are isoforms of BCR (PMID : 2494632, PMID : 3078961).

All lanes:

Immunoprecipitation - Anti-Bcr antibody [EPR22062] (ab222406)

Predicted band size: 143 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcr antibody [EPR22062] (AB222406)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcr antibody [EPR22062] (AB222406)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Bcr with ab222406 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in rat cerebrum (PMID : 25331951). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcr antibody [EPR22062] (AB222406)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Bcr with ab222406 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in mouse hippocampus (PMID : 25331951). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

Supplier Data

Western blot - Anti-Bcr antibody [EPR22062] (AB222406)

Blocking/Dilution buffer : 5% NFDM/TBST.

The 230/210 kDa band is the BCR-ABL fusion protein in K562 cells (PMID : 17684099, PMID : 16585609, PMID : 12476301). The 160 kDa and 130 kDa bands are isoforms of BCR (PMID : 2494632, PMID : 3078961).

All lanes:

Western blot - Anti-Bcr antibody [EPR22062] (ab222406) at 1/1000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole lysate at 20 µg

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embyro fibroblast cell line) whole lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 143 kDa

Observed band size: 130 kDa,160 kDa,210 kDa,230 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Bcr antibody [EPR22062] (AB222406)

Exposure time : Lanes 1 and 2 : 3 minutes; Lanes 3 and 4 : 59 seconds; Lane 3 : 81 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The 160 kDa and 130 kDa bands are isoforms of BCR (PMID : 2494632, PMID : 3078961).

All lanes:

Western blot - Anti-Bcr antibody [EPR22062] (ab222406) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole lysate at 20 µg

Lane 2:

Human brain lysate at 20 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole lysate at 20 µg

Lane 4:

Rat brain lysate at 20 µg

Lane 5:

Human testis lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 143 kDa

Observed band size: 130 kDa,160 kDa

false

Lab

Western blot - Anti-Bcr antibody [EPR22062] (AB222406)

Lanes 1 - 2 : Merged signal (red and green). Green - ab222406 observed at 150 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

ab222406 was shown to react with BCR in wild-type HEK-293T cells in western blot. The bands observed in BCR CRISPR/Cas9 edited cell line ab266583 (BCR CRISPR/Cas9 edited cell lysate ab257858) below 150kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and BCR CRISPR/Cas9 edited HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab222406 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4&

176;C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Bcr antibody [EPR22062] (ab222406) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

BCR CRISPR/Cas9 edited HEK-293T cell lysate at 20 µg

Predicted band size: 143 kDa

Observed band size: 150 kDa

false

Supplier Data

Western blot - Anti-Bcr antibody [EPR22062] (AB222406)

All lanes:

Western blot - Anti-Bcr antibody [EPR22062] (ab222406) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

BCR knockout HEK-293T cell lysate at 20 µg

Predicted band size: 143 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22062

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

IHC is not recommended for human.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Bcr protein also known as breakpoint cluster region protein plays an important role in cellular signaling. It has a molecular mass of approximately 160 kDa and is expressed in many cell types such as hematopoietic cells. Bcr functions as a GTPase-activating protein (GAP) for p21rac and other small GTP-binding proteins. This activity influences cellular processes such as growth and differentiation. Bcr is also known for its involvement in the formation of the Bcr-Abl fusion protein an important factor in certain leukemias.

Biological function summary

The Bcr protein acts within cells to regulate signal transduction and cytoskeletal organization. It is part of the pre-B cell receptor (pre-BCR) signaling complex influencing B-cell development. Its GAP activity is important for the regulation of small GTPases impacting cell migration and adhesion. Alterations in Bcr function can affect cellular responses such as apoptosis making it a focus for research into targeted therapies for hematological conditions.

Pathways

The Bcr protein is an important player in signaling pathways involving the Ras superfamily of small GTPases. It is notably involved in the Rho GTPase pathway tied to cytoskeletal dynamics and cell cycle progression. Bcr's interaction with these pathways puts it in relation to proteins like Rac and Cdc42 which are vital for various cellular processes. Disruption in Bcr-associated pathways can lead to aberrant cell growth and metastasis.

Bcr has a well-documented link to certain leukemias particularly chronic myeloid leukemia (CML). The Bcr-Abl fusion protein resulting from the translocation of chromosomes 9 and 22 is central to CML pathology. Additionally Bcr connects with other proteins such as Abl in this disease context. Further disruptions in Bcr signaling can relate to disorders involving immune system dysregulation demonstrating the protein's relevance in both oncology and immunology research.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form (PubMed : 17116687, PubMed : 1903516, PubMed : 7479768). The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form (PubMed : 23940119, PubMed : 7479768). The amino terminus contains an intrinsic kinase activity (PubMed : 1657398). Functions as an important negative regulator of neuronal RAC1 activity (By similarity). Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity (PubMed : 17116687). Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation (PubMed : 23940119).

See full target information BCR

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40867839

2025

Biomarker-Driven Optimization of Saponin Therapy in MASLD: From Mouse Models to Human Liver Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Young Kim,Ju Hee Oh,Hyun Sung Kim,Dae Won Jun

Aging 12:2595-2609 PubMed32028262

2020

Notch1/2/3/4 are prognostic biomarker and correlated with immune infiltrates in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hu,Jianghong Yu,Jun Gan,Ning Song,Liubin Shi,Jie Liu,Ziqiang Zhang,Jianjun Du

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bcr antibody [EPR22062]

Flow Cytometry (Intracellular) - Anti-Bcr antibody [EPR22062] (AB222406)

Immunoprecipitation - Anti-Bcr antibody [EPR22062] (AB222406)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcr antibody [EPR22062] (AB222406)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcr antibody [EPR22062] (AB222406)

Western blot - Anti-Bcr antibody [EPR22062] (AB222406)

Western blot - Anti-Bcr antibody [EPR22062] (AB222406)

Western blot - Anti-Bcr antibody [EPR22062] (AB222406)

Western blot - Anti-Bcr antibody [EPR22062] (AB222406)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise