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AB213323

Anti-BDNF antibody [EPR21211]

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(10 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal BDNF antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 10 publications.

대체 명칭 보기

Neurotrophic factor BDNF precursor form, proBDNF, Abrineurin, Brain-derived neurotrophic factor, BDNF

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR21211] (AB213323)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR21211] (AB213323)

IHC image of BDNF staining in normal rat brain dentate gyrus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab213323, 0.05 μg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR21211] (AB213323)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR21211] (AB213323)

IHC image of BDNF staining in normal mouse brain dentate gyrus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab213323, 0.05μg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21211

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB203573

Anti-BDNF antibody [3C11]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [3C11] (AB203573)

  • 1
  • 1
Primary Antibodies

AB222321

Alexa Fluor® 647 Anti-BDNF antibody [EPR1292]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-BDNF antibody [EPR1292] (AB222321)

  • 0
  • 0
Primary Antibodies

AB108319

Anti-BDNF antibody [EPR1292]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-BDNF antibody [EPR1292] (AB108319)

  • 4
  • 10
Primary Antibodies

AB216443

Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (AB216443)

  • 0
  • 0
Primary Antibodies

AB10505

Anti-BDNF antibody [35928.11]

  • 4
  • 1
Primary Antibodies

AB205067

Anti-BDNF antibody [3B2]

Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [3B2] (AB205067)

  • 0
  • 0
Primary Antibodies

AB226843

Anti-BDNF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (AB226843)

  • 0
  • 0
Primary Antibodies

AB223354

Anti-BDNF antibody

Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (AB223354)

  • 0
  • 0
Primary Antibodies

AB271873

Anti-BDNF antibody [EPR1292] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (AB271873)

  • 0
  • 0
Primary Antibodies

AB222113

Alexa Fluor® 488 Anti-BDNF antibody [EPR1292]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-BDNF antibody [EPR1292] (AB222113)

  • 0
  • 0

보완 제품

Assay Kits

AB212166

Human BDNF ELISA Kit

SimpleStep

ELISA - Human BDNF ELISA Kit (AB212166)

  • 4
  • 2
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 75
Primary Antibodies

AB278054

Anti-GFAP antibody [RM1003] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [RM1003] - Astrocyte Marker (AB278054)

  • 0
  • 0
Primary Antibodies

AB192757

Anti-PSD95 antibody [K28/43] - Synaptic Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PSD95 antibody [K28/43] - Synaptic Marker (AB192757)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.01-0.05 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.01-0.05 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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제품 세부 정보

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

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특성 및 보관 정보

제형
Liquid
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed : 11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.. Neurotrophic factor BDNF precursor form. Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed : 24908487, PubMed : 29909994). Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).
See full target information BDNF
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제품이 사용된 논문 (10)

Recent publications for all applications. Explore the 전체 목록 and refine your search

eNeuro : PubMed40064496

2025

Electroacupuncture Neural Stimulation Mitigates Bladder Dysfunction and Mechanical Allodynia in Cyclophosphamide Induced Cystitis through Downregulation of the BDNF-TrkB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Su,Fei Yang,Jun-Cong Xie,Chi Zhang,Rui-Xiang Luo,Wen-Shuang Li,Bo-Long Liu,Min-Zhi Su

BMC neuroscience 25:36 PubMed39103771

2024

Intracerebroventricular administration of the exercise hormone irisin or acute strenuous exercise alleviates epileptic seizure-induced neuroinflammation and improves memory dysfunction in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zarife Nigâr Ozdemir-Kumral,Tuğçe Akgün,Ceren Haşim,Ezgi Ulusoy,Mehmet Kaan Kalpakçıoğlu,Muhammet Ferzan Yüksel,Tunahan Okumuş,Zeynep Us,Dilek Akakın,Meral Yüksel,Zafer Gören,Berrak Ç Yeğen

The Journal of nutrition 154:2752-2762 PubMed39053605

2024

Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Imani Muhammad,Eleonora Cremonini,Patricia Mathieu,Ana M Adamo,Patricia I Oteiza

Theranostics 13:1059-1075 PubMed36793868

2023

Hyperactive neuronal autophagy depletes BDNF and impairs adult hippocampal neurogenesis in a corticosterone-induced mouse model of depression.

Applications

Unspecified application

Species

Unspecified reactive species

Kuo Zhang,Fan Wang,Mengying Zhai,Meiyao He,Yuxuan Hu,Lijin Feng,Yuting Li,Jingyu Yang,Chunfu Wu

Physiological reports 11:e15562 PubMed36636750

2023

Restraint stress during neonatal hypoxia-ischemia alters brain injury following normothermia and hypothermia.

Applications

Unspecified application

Species

Unspecified reactive species

Julia K Gundersen,Hemmen Sabir,Thomas R Wood,Damjan Osredkar,Mari Falck,Else M Loeberg,Lars Walloe,David A Menassa,Marianne Thoresen

Histology and histopathology 37:555-573 PubMed35191013

2022

Maternal exposure to the environmental pollutant "BDE-47" impairs the postnatal development of rat cerebellar cortex by modulating neuronal proliferation, synaptogenesis, NGF and BDNF pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Dalia A Mandour,Asmaa M Tolba,Emtethal M El-Bestawy

Journal of clinical medicine 10: PubMed34768320

2021

Is Saffron Able to Prevent the Dysregulation of Retinal Cytokines Induced by Ocular Hypertension in Mice?

Applications

Unspecified application

Species

Unspecified reactive species

José A Fernández-Albarral,Miguel A Martínez-López,Eva M Marco,Rosa de Hoz,Beatriz Martín-Sánchez,Diego San Felipe,Elena Salobrar-García,Inés López-Cuenca,María D Pinazo-Durán,Juan J Salazar,José M Ramírez,Meritxell López-Gallardo,Ana I Ramírez

Genomics, proteomics & bioinformatics 20:657-669 PubMed33647481

2021

High VHL Expression Reverses Warburg Phenotype and Enhances Immunogenicity in Kidney Tumor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Songbiao Zhu,Wenxi Ding,Yuling Chen,Weixuan Wang,Renhua Xu,Chongdong Liu,Xiaohui Liu,Haiteng Deng

International journal of molecular sciences 22: PubMed33669765

2021

Retinal Molecular Changes Are Associated with Neuroinflammation and Loss of RGCs in an Experimental Model of Glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

José A Fernández-Albarral,Juan J Salazar,Rosa de Hoz,Eva M Marco,Beatriz Martín-Sánchez,Elena Flores-Salguero,Elena Salobrar-García,Inés López-Cuenca,Vicente Barrios-Sabador,Marcelino Avilés-Trigueros,Francisco J Valiente-Soriano,Juan A Miralles de Imperial-Ollero,Manuel Vidal-Sanz,Alberto Triviño,José M Ramírez,Meritxell López-Gallardo,Ana I Ramírez

Journal of neuroinflammation 17:19 PubMed31931832

2020

BDNF promotes activation of astrocytes and microglia contributing to neuroinflammation and mechanical allodynia in cyclophosphamide-induced cystitis.

Applications

Unspecified application

Species

Unspecified reactive species

Honglu Ding,Jialiang Chen,Minzhi Su,Zhijun Lin,Hailun Zhan,Fei Yang,Wenbiao Li,Juncong Xie,Yong Huang,Xianguo Liu,Bolong Liu,Xiangfu Zhou
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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