AB215889

Anti-beta 2 Microglobulin antibody [B2M/961]

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(2 제품이 사용된 논문 )

Mouse Monoclonal beta 2 Microglobulin antibody. Suitable for ICC, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human B2M.

대체 명칭 보기

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin

8 이미지

Supplier Data

Immunocytochemistry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunocytochemistry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling beta 2 Microglobulin with ab215889 at 4 μg/mL followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling beta 2 Microglobulin with ab215889 at 2 μg/10⁶ cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrial carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bladder carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

Supplier Data

Western blot - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

All lanes:

Western blot - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889) at 2 µg/mL

Lane 1:

THP-1 (human monocytic leukemia cell line) whole cell lysate

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate

Predicted band size: 14 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

B2M/961

Isotype

IgG2b

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

ICC, Flow Cyt, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human B2M.

P61769

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-4 µg/mL", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Primary incubation for 30 minutes at room temperature." }, "Primates": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

ab215889 was purified from Bioreactor Concentrate by Protein A/G.

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.

Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed : 25356553).

See full target information B2M

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JCI insight 8: PubMed36946463

2023

JAK-STAT activation contributes to cytotoxic T cell-mediated basal cell death in human chronic lung allograft dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Aaditya Khatri,Jamie L Todd,Fran L Kelly,Andrew Nagler,Zhicheng Ji,Vaibhav Jain,Simon G Gregory,Kent J Weinhold,Scott M Palmer

Nature communications 9:3634 PubMed30194383

2018

Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel B Ferguson,Ben Van Handel,Maxwell Bay,Petko Fiziev,Tonis Org,Siyoung Lee,Ruzanna Shkhyan,Nicholas W Banks,Mila Scheinberg,Ling Wu,Biagio Saitta,Joseph Elphingstone,A Noelle Larson,Scott M Riester,April D Pyle,Nicholas M Bernthal,Hanna Ka Mikkola,Jason Ernst,Andre J van Wijnen,Michael Bonaguidi,Denis Evseenko

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta 2 Microglobulin antibody [B2M/961]

Immunocytochemistry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Western blot - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise