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AB314186

Anti-beta Casein antibody [29D6]

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Rabbit Recombinant Monoclonal beta Casein antibody. Suitable for IHC-P, Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CSN2.

대체 명칭 보기

CASB, CSN2, Beta-casein

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Casein antibody [29D6] (AB314186)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Casein antibody [29D6] (AB314186)

Immunohistochemical analysis of ab314186 diluted at 1 : 100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Casein antibody [29D6] (AB314186)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Casein antibody [29D6] (AB314186)

Immunohistochemical analysis of ab314186 diluted at 1 : 100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Flow Cytometry - Anti-beta Casein antibody [29D6] (AB314186)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-beta Casein antibody [29D6] (AB314186)

Overlay Peak curve showing HepG2 (Human liver hepatocellular carcinoma cell line) cells stained with ab314186 (pink line) at 1 : 100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1x10E6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1 : 200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1ug/1x10E6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

29D6

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Synthetic Peptide within Human CSN2.

P05814

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보완 제품

Primary Antibodies

AB178431

Anti-alpha Lactalbumin antibody [EPR12460]

RabMAb|Recombinant

Western blot - Anti-alpha Lactalbumin antibody [EPR12460] (AB178431)

  • 0
  • 0
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB166596

Anti-Casein antibody

Western blot - Anti-Casein antibody (AB166596)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/200", "FlowCyt-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Beta-casein is a major component of milk proteins known also simply as casein or beta-casein protein. This protein plays an important structural role in forming micelles which ensures efficient transport and digestion of calcium and phosphate in milk. Beta-casein has a molecular weight of about 24 kDa. It is primarily expressed in the mammary gland produced during lactation. Analyzing beta-casein often involves techniques like casein SDS PAGE which helps in confirming its molecular mass and purity.
Biological function summary

Beta-casein is involved in both nutritional and protective functions. In the mammary gland it is part of the casein micelle complex which includes other caseins like alpha-casein and kappa-casein. These micelles improve the stability and solubility of milk proteins. Beta-casein’s phosphorylation allows binding with calcium ions supporting skeletal development. This function is significant in neonatal growth as casein protein delivers amino acids critical for development.

Pathways

Beta-casein functions within pathways related to calcium transport and absorption. It interacts with proteins involved in the signaling pathways such as osteopontin enhancing mineral absorption. In milk production processes beta-casein supports micelle assembly and stabilization forming complexes that incorporate other milk proteins. These interactions are essential in ensuring the proper delivery of nutrients and minerals to developing offspring.

Beta-casein may play a role in dairy-related sensitivities and certain metabolic disorders. Some studies suggest its A1 variant could be linked to digestive discomfort in lactose-intolerant individuals. Beta-casein’s protein fragments such as beta-casomorphin are also under investigation for links to conditions like type 1 diabetes. In these contexts beta-casein's interaction with proteins involved in immune response might influence disease progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Important role in determination of the surface properties of the casein micelles.
See full target information CSN2
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Product promise

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