Anti-beta Catenin antibody [SP328]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-beta Catenin antibody [SP328] (AB224803)

Flow cytometric analysis of HEK-293 (human epithelial cell line from embryonic kidney) cell line labeling beta Catenin with ab224803 at 1/400 dilution (green) compared to a negative control of rabbit IgG (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human lung tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human colon tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human bladder tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human ovary adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human ovary tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human prostate tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human bladder transitional carcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human kidney tissue stained for beta Catenin withab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling beta Catenin with ab224803 at 1/100 dilution (1.20 μg/ml). Heat mediated antigen retrieval with sodium Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224803 for 10 mins at room temperature.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human lung squamous carcinoma tissue stained for beta Catenin withab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-beta Catenin antibody [SP328] (AB224803)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling beta Catenin with purified ab224803 at 1/200 dilution (0.495 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human liver tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [SP328] (AB224803)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling beta Catenin with purified ab224803 at 1/10 (9.9 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling beta Catenin with ab224803 at 1/100 dilution (1.20 μg/ml). Heat mediated antigen retrieval with sodium Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly membranous with nuclear staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224803 for 10 mins at room temperature.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human stomach tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Formalin-fixed, paraffin-embedded human breast tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

• IP

Lab

Immunoprecipitation - Anti-beta Catenin antibody [SP328] (AB224803)

beta Catenin was immunoprecipitated from HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab224803 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab224803 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-beta Catenin antibody [SP328] (ab224803) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (Input) at 10 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (-)

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab224803 in HeLa whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 85 kDa

false

Exposure time: 3s

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [SP328] (AB224803)

Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling beta Catenin with purified ab224803 at 1/10 (9.9 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-beta Catenin antibody [SP328] (AB224803)

Flow cytometry analysis of NIH/3T3 (mouse embryonic fibroblast) labeling beta Catenin with purified ab224803 at 1/200 dilution (0.495 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control -Unlabelled cells (blue).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-beta Catenin antibody [SP328] (AB224803)

Flow cytometry analysis of C6 (rat glial tumor glial cell) labeling beta Catenin with purified ab224803 at 1/200 dilution (0.495 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse pancreas tissue sections labeling beta Catenin with ab224803 at 1/100 dilution (1.20 μg/ml). Heat mediated antigen retrieval with sodium Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly membranous staining on the mouse pancreas, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224803 for 10 mins at room temperature.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [SP328] (AB224803)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat pancreas tissue sections labeling beta Catenin with ab224803 at 1/100 dilution (1.20 μg/ml). Heat mediated antigen retrieval with sodium Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly membranous staining on the rat pancreas, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224803 for 10 mins at room temperature.

• IP

Lab

Immunoprecipitation - Anti-beta Catenin antibody [SP328] (AB224803)

beta Catenin was immunoprecipitated from Rat brain tissue lysate with ab224803 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab224803 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-beta Catenin antibody [SP328] (ab224803) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate (Input) at 10 µg

Lane 2:

Rat brain tissue lysate (+)

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/proteins-peptides/recombinant-phosphinotricin-acetyl-transferase-protein-his-tag-ab224834'>ab224834</a> in rat brain tissue lysate (-)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 85 kDa

false

Exposure time: 3s

• IP

Lab

Immunoprecipitation - Anti-beta Catenin antibody [SP328] (AB224803)

beta Catenin was immunoprecipitated from Mouse brain tissue lysate with ab224803 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab224803 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-beta Catenin antibody [SP328] (ab224803) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate (Input) at 10 µg

Lane 2:

Mouse brain tissue lysate (+)

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab224803 in mouse brain tissue lysate (-)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 85 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-beta Catenin antibody [SP328] (AB224803)

False colour image of Western blot : Anti-beta Catenin antibody [SP328] staining at 1/400 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab224803 was shown to bind specifically to beta Catenin. A band was observed at 85/90 kDa in wild-type MCF7 cell lysates with no signal observed at this size in CTNNB1 knockout cell line ab286762. To generate this image, wild-type and CTNNB1 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-beta Catenin antibody [SP328] (ab224803) at 1/400 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

CTNNB1 knockout MCF7 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 85 kDa

Observed band size: 85/90 kDa

false

• WB

Supplier Data

Western blot - Anti-beta Catenin antibody [SP328] (AB224803)

All lanes:

Western blot - Anti-beta Catenin antibody [SP328] (ab224803) at 1/100 dilution

All lanes:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 85 kDa

false