Anti-beta III Tubulin antibody [2G10] - Neuronal Marker

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TUBB3 KO HeLa (ab255358) / wild-type HeLa cells labelling beta III Tubulin with ab78078 at 1/200 dilution followed by a secondary antibody ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in parental HeLa cell line, and no staining in TUBB3 HeLa KO cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counterstain beta Tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) and Differentiated SH-SY5Y cells labelling KCNQ2 with ab325971 at 1/50 (10.26 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in differentiated SH-SY5Y cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used to counterstain tubulin at 1/1000 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-P

AbReview16430****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunohistochemistical detection of Neuron specific beta III Tubulin antibody [2G10] using ab78078 in formaldehyde-fixed paraffin-embedded quail embryo eye sections. Antigen retrieval step : heat mediated in citric acid pH6 buffer. Blocking step : 1% BSA for 10 mins @ rt°C Primary antibody incubated at 1/1250 for 2 hours in TBS/BSA/azide. Secondary antibody : anti-Mouse IgG conjugated to biotin used at 1/200. In this image, the lining cells of the hyaloid artery are positive. Excellent neural component specificity : even small fibres outside of the cartilage model of the orbit are clearly demonstrated. This image was generated using a previous batch manufactured using hybridoma production method

This image is courtesy of Carl Hobbs, King's College London, United Kingdom

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) and Differentiated SH-SY5Y cells labelling JIP3 with ab326560 at 1/100 (5.12 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing increased cytoplasmic staining in differentiated SH-SY5Y cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) Neuronal differentiated SH-SY5Y labelling Tau (MBD region) with ab308439 at 1/50 (9.64 μg/mL) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 2 μg/mL dilution (Green). Confocal image showing increased cytoplasmic staining in neurnal differentiated SH-SY5Y cells (shown in green). ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker at 1/200 dilution (2.5 μg/ml) was used as a counterstain observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human iPSC derived ioSensory Neurons (ab317489) cells labelling Nav1.8/SCN10A with ab326327 at 1/200 (2.405 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in human iPSC derived ioSensory neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used to counterstain tubulin at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution(Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) Neuronal differentiated SH-SY5Y cells labelling with ab327082 at 1/50 (10.06 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing increased cytoplasmic staining in neurnal differentiated SH-SY5Y cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) neural differentiated SH-SY5Y cells labelling with ab327002 at 1/200 (2.43 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing increased cytoplasmic and nuclear staining in neural differentiated SH-SY5Y cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used to counterstain tubulin at 1/500 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human iPSC derived ioSensory Neurons (ab317489) cells labelling CGRP-I with ab327004 at 1/50 (10.26 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in human iPSC derived ioSensory Neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) Differentiated SH-SY5Y labelling DNAJC6 with ab289711 at 1/200 (2.275 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody as secondary at 1/1000 (2 μg/ml) dilution. ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used as a primary target counterstain used at 1/200 (2.5 μg/ml) along with ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) used at 1/1000 (2μg/ml) as secondary.

Confocal image showing increased cytoplasmic staining in differentiated SH-SY5Y cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labelling beta III Tubulin with ab78078 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cerebellum.
The section was incubated with ab78078 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse primary DRG neuron cells labelling CGRP-I with ab327004 at 1/50 (10.26 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in mouse primary DRG neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat primary DRG neuron cells labelling CGRP-I with ab327004 at 1/50 (10.26 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in rat primary DRG neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde fixed rat DRG primary neurons permeabilized using 0.1% Triton X-100 labelling TRPV1 with ab305299 (green) followed by ab150081 AlexaFluor^®488 preadsorbed Goat anti-Rabbit secondary. ab78078 anti-beta III Tubulin (mouse mAb) was used as counterstain (magenta) with ab150120 AlexaFluor^®594 Goat anti-Mouse as secondary. Nuclear DNA was labelled with DAPI (shown in blue). Image showing cytoplasmic staining in rat DRG primary neurons was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cell labelling beta III Tubulin with ab78078 at 1/1000 dilution (0.1µg)/ Right compared with a Mouse monoclonal IgG Isotype Control Antibody /Left.

Goat anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cell labelling beta III Tubulin with ab78078 at 1/1000 dilution (0.1µg)/ Right compared with a Mouse monoclonal IgG Isotype Control Antibody /Left.

Goat anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling beta III Tubulin with ab78078 at 1/200 dilution followed by a secondary antibody ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green).

Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue).

ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain MAP2 at 1/1000 dilution followed by a secondary antibody ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labelling beta III Tubulin with ab78078 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cerebellum.
The section was incubated with ab78078 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling beta III Tubulin with ab78078 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebellum.
The section was incubated with ab78078 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Soft palate innervation is primarily oro-medial and complements the differentiated muscle pattern

(A-L) MHC (green) and β3-tubulin (red) co-immunostaining along the antero-posterior axis of the mouse soft palate at E13.5 (A-C), E14.5 (D-F), E16.5 (G-I), and P0 (J-L) at the level of the TVP and PLG (A, D, G, J), LVP (B, E, H, K), and PLP and SPC (C, F, I, L). Dashed lines indicate the outline of the palatal shelf and tongue epithelium. Arrows indicate the nerve fibers innervating the palatal shelves in a pattern complementary to the differentiated muscle, mainly located orally in the medial region. The schematic drawings indicate the orientation and the position of each section. Scale bars : 200μm.

beta III Tubulin is detected with ab78078.

(From Figure 4 of Grimaldi et al)

This image was generated using a previous batch manufactured using hybridoma production method

Grimaldi, A. et al PLoS One. 2015 Dec 15;10(12):e0145018. doi: 10.1371/journal.pone.0145018. eCollection 2015. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

• ICC/IF

AbReview48553****

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

ab78078 staining beta III Tubulin (red) in mouse differentiated neural stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100. Samples were incubated with primary antibody (5μg/ml in PBS + 3% BSA) for 16 hours at 4°C. An Alexa Fluor^® 568-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Blue - DAPI nuclear counterstain. This image was generated using a previous batch manufactured using hybridoma production method

This image is courtesy of an anonymous Abreview

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary DRG neuron cells labelling Nav1.8/SCN10A with ab307817 at 1/100 (5.32 μg/ml) (shown in Green).

Confocal image showing cytoplasmic staining in rat primary DRG neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution, followed by secondary counterstain ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution.

Secondary ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was used at 1/1000 (2μg/ml)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde fixed mouse DRG primary neurons permeabilized using 0.1% Triton X-100 labelling CGRP-I+CGRP-II with ab283568 (green) followed by ab150081 AlexaFluor^®488 preadsorbed Goat anti-Rabbit secondary. ab78078 anti-beta III Tubulin (mouse mAb) was used as counterstain (magenta) with ab150120 AlexaFluor^®594 Goat anti-Mouse as secondary. Nuclear DNA was labelled with DAPI (shown in blue). Image showing cytoplasmic staining in mouse DRG primary neurons was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labelling beta III Tubulin with ab78078 at 1/200 dilution followed by a secondary antibody ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green).

Confocal image showing positive staining in rat primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue).

ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain MAP2 at 1/1000 dilution followed by a secondary antibody ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse DRG (Mouse primary dorsal root ganglion cell) cells labelling SHOX2 with ab326071 at 1/200 (2.51 µg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing mainly nuclear staining in mouse DRG (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.

ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab326071 at 1/200 dilution, followed by ab150120 at 1/1000 dilution.-ve control 2 : ab78078 at 1/500 dilution, followed by ab150081 at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde fixed rat DRG primary neurons permeabilized using 0.1% Triton X-100 labelling CGRP-I+CGRP-II with ab283568 (green) followed by ab150081 AlexaFluor^®488 preadsorbed Goat anti-Rabbit secondary. ab78078 anti-beta III Tubulin (mouse mAb) was used as counterstain (magenta) with ab150120 AlexaFluor^®594 Goat anti-Mouse as secondary. Nuclear DNA was labelled with DAPI (shown in blue). Image showing cytoplasmic staining in rat DRG primary neurons was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunofluorescent analysis of 4% Paraformaldehyde fixed mouse DRG primary neurons permeabilized using 0.1% Triton X-100 labelling TRPV1 with ab305299 (green) followed by ab150081 AlexaFluor^®488 preadsorbed Goat anti-Rabbit secondary. ab78078 anti-beta III Tubulin (mouse mAb) was used as counterstain (magenta) with ab150120 AlexaFluor^®594 Goat anti-Mouse as secondary. Nuclear DNA was labelled with DAPI (shown in blue). Image showing cytoplasmic staining in mouse DRG primary neurons was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IP

Lab

Immunoprecipitation - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

beta III Tubulin was immunoprecipitated from Mouse brain tissue lysate 10 µg with ab78078 at 1/50 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab78078 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) at 1/50 dilution

Lane 1:

Mouse brain tissue lysate (Input) at 10 µg

Lane 2:

Mouse brain tissue lysate (+)

Lane 3:

Mouse IgG1, kappa monoclonal isotype control (<a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18443'>ab18443</a>) instead of ab78078 in mouse brain tissue lysate (-)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/ko/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Observed band size: 50 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

False colour image of Western blot : Anti-beta III Tubulin antibody [2G10] staining at 1/1000 dilution, shown in green; Rabbit anti-GAPDH antibody ab181602 loading control staining at 1/20000 dilution, shown in red.

In Western blot, ab78078 was shown to bind specifically to beta III Tubulin. A band was observed at 50 kDa in wild-type HeLa cell lysates with no signal observed at this size in the beta III Tubulin knockout cell line. To generate this image, wild-type and beta III Tubulin knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a PVDF-FL membrane. Membranes were blocked in Odyssey diluted in equal volume of 0.1 % TBS before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Mouse IgG H&L (IRDye^® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) (ab216777) at 1/10000 dilution.

All lanes:

Western blot - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) at 1/1000 dilution

Lane 1:

Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

TUBB3 knockout HeLa whole cell lysate at 20 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/10000 dilution

Lanes 1 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

Observed band size: 50 kDa

false

• WB

Lab

Western blot - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure Time :
Lane 1 : 136 seconds
Lane 2,3 : 15 seconds

All lanes:

Western blot - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) at 1/2000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/50000 dilution

Observed band size: 50 kDa

false

• IHC-P

AbReview17595****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunohistochemistical detection of Neuron specific beta III Tubulin antibody using ab78078 on formaldehyde-fixed paraffin-embedded marmoset cerebellum sections. Antigen retrieval step : Heat mediated in Citric acid pH6 buffer. Permeabilization : No. Blocking step : 1% BSA for 10 mins @ rt°C. Primary Antibody used at 1/1000 incubated for 2 hours in TBS/BSA/azide. Secondary Antibody : anti mouse IgG conjugation to biotin (1/200).

This image is courtesy of Carl Hobbs, King's College London, United Kingdom

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

Immunohistochemical detection of Neuron specific beta III Tubulin using ab78078 on formaldehyde fixed dogfish/catshark PNS tissue sections. Antigen retrieval step : heat mediated in citric acid. Blocking : 1% BSA for 10 mins @ rt°C. Primary antibody ab78078 incubated at 1/1250 for 2 hours. Secondary Antibody : anti mouse IgG conjugated to biotin @ 1/200 This is a composite image of neuronal cell bodies and fibres; the upper image uses coloured arrowheads to indicate positive PNS components : cell bodies (black), L/S (green) and T/S (red) nerve fibres of what appear to be three Ganglia. Note that the L/S fibres do not appear as well-stained as the T/S fibres. The lower image shows what appears to be a linear sequence of single nerve cells and their processes within the core of a gill. This image was generated using a previous batch manufactured using hybridoma production method

This image is courtesy of Carl Hobbs, King's College London, United Kingdom