Anti-BHLHE40 Antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-BHLHE40 Antibody (AB327215)

Immunofluorescent staining of human cell line U-251 MG using ab327215 at 0.25–2 µg/ml, showing localization to nuclear bodies.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHLHE40 Antibody (AB327215)

Immunohistochemical staining of human pancreas using ab327215 at 1 : 50–1 : 200 dilution, showing moderate cytoplasmic positivity in exocrine glandular cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHLHE40 Antibody (AB327215)

Immunohistochemical staining of human duodenum using ab327215 at 1 : 50–1 : 200 dilution, showing moderate cytoplasmic positivity in glandular cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHLHE40 Antibody (AB327215)

Immunohistochemical staining of human prostate using ab327215 at 1 : 50–1 : 200 dilution, showing moderate cytoplasmic positivity in smooth muscle cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHLHE40 Antibody (AB327215)

Immunohistochemical staining of human urinary bladder using ab327215 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in urothelial cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHLHE40 Antibody (AB327215)

Immunohistochemical staining of human esophagus using ab327215 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in squamous epithelial cells.

• ChIP

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ChIP - Anti-BHLHE40 Antibody (AB327215)

ChIP-Exo-Seq composite graph for ab327215 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

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Western blot - Anti-BHLHE40 Antibody (AB327215)

Western blot analysis in human cell line HDLM-2 using ab327215 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-BHLHE40 Antibody (ab327215)

All lanes:

Human cell line HDLM-2

Predicted band size: 45.51 kDa

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