AB27553

Biotin Anti-Cytokeratin 18 antibody [C-04]

Name: Biotin Anti-Cytokeratin 18 antibody [C-04]
Brand: Abcam Limited
SKU: AB27553
Rating: 5 (2 reviews)

(2 리뷰들)

(6 제품이 사용된 논문 )

Anti-Cytokeratin 18 antibody [C-04] conjugated to Biotin - suitable for Western blot, and reacts with human samples. Clone [C-04] is a mouse monoclonal antibody cited in 10 publications.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

C-04

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Cell preparation containing KRT18 protein. The exact immunogen used to generate this antibody is proprietary information.

P05783

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Size-exclusion chromatography

Purification 관련 사항

Purity >95% by SDS-PAGE.

보관 버퍼

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed : 26823019). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (PubMed : 26823019). Organization and orientation of KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

대체 명칭 보기

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature genetics 57:2177-2191 PubMed40858905

2025

ERG-driven prostate cancer initiation is cell-context dependent and requires KMT2A and DOT1L.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Feng,Erik Ladewig,Matthew Lange,Nazifa Salsabeel,Huiyong Zhao,Young Sun Lee,Anuradha Gopalan,Hanzhi Luo,Wenfei Kang,Ning Fan,Eric Rosiek,Elisa de Stanchina,Yu Chen,Brett S Carver,Christina S Leslie,Charles L Sawyers

Cell reports 41:111641 PubMed36384107

2022

A dietary change to a high-fat diet initiates a rapid adaptation of the intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Jacob R Enriquez,Heather A McCauley,Kevin X Zhang,J Guillermo Sanchez,Gregory T Kalin,Richard A Lang,James M Wells

Oncotarget 8:93899-93911 PubMed29212197

2017

Paeoniflorin prevents postoperative peritoneal adhesion formation in an experimental rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Gao,Guangbing Wei,Yunhua Wu,Na Yao,Cancan Zhou,Kai Wang,Kang Wang,Xuejun Sun,Xuqi Li

Nature cell biology 16:942-50, 1-7 PubMed25173976

2014

Mammary stem cells have myoepithelial cell properties.

Applications

IHC-P

Species

Mouse

Michael D Prater,Valérie Petit,I Alasdair Russell,Rajshekhar R Giraddi,Mona Shehata,Suraj Menon,Reiner Schulte,Ivo Kalajzic,Nicola Rath,Michael F Olson,Daniel Metzger,Marisa M Faraldo,Marie-Ange Deugnier,Marina A Glukhova,John Stingl

PloS one 8:e51671 PubMed23300950

2013

FGFR2 promotes breast tumorigenicity through maintenance of breast tumor-initiating cells.

Applications

IHC-P

Species

Mouse

Sungeun Kim,Anna Dubrovska,Richard J Salamone,John R Walker,Kathryn B Grandinetti,Ghislain M C Bonamy,Anthony P Orth,Jimmy Elliott,Diana Graus Porta,Carlos Garcia-Echeverria,Venkateshwar A Reddy

Stem cells (Dayton, Ohio) 26:2245-56 PubMed18556507

2008

Scalable selection of hepatocyte- and hepatocyte precursor-like cells from culture of differentiating transgenically modified murine embryonic stem cells.

Applications

ICC/IF

Species

Unspecified reactive species

Irina Drobinskaya,Thomas Linn,Tomo Saric,Reinhard G Bretzel,Heribert Bohlen,Jürgen Hescheler,Eugen Kolossov

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Cytokeratin 18 antibody [C-04]

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise