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AB317078

Biotin Anti-Cytokeratin 18 antibody [SP69]

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Rabbit Recombinant Monoclonal Cytokeratin 18 antibody - conjugated to Biotin. Suitable for IHC-P, WB and reacts with Human samples.

대체 명칭 보기

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Cytokeratin 18 antibody [SP69] (AB317078)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Cytokeratin 18 antibody [SP69] (AB317078)

IHC image of Cytokeratin 18 staining in a section of formalin-fixed paraffin-embedded human normal endometrium*.

Performed on a Leica BONDTM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab317078 at 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

관련 conjugated 항체와 다양한 조성의 항체 (2)

  • Unconjugated

    Anti-Cytokeratin 18 antibody [SP69]

  • Carrier free

    Anti-Cytokeratin 18 antibody [SP69] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP69

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB254515

Human Cytokeratin 18 Fragment ELISA Kit (CK18-30)

Recombinant|SimpleStep

ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)

  • 0
  • 0
Primary Antibodies

AB192643

Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y]

RabMAb|Recombinant|KO Validated

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192643)

  • 4
  • 1
Primary Antibodies

AB192467

Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192467)

  • 5
  • 5
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB185048

Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB185048)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.
Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed : 26823019). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (PubMed : 26823019). Organization and orientation of KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
See full target information KRT18
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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