Biotin Anti-GFP antibody

• IHC-P

AbReview16271****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GFP antibody (AB6658)

ab6658 staining GFP in human melanoma cells recovered from nude mice by Immunocytochemistry/ immunoflurescence. Cells were fixed with formaldehyde, permeabilized with 0.25% Triton X-100 RT for 10min and blocking with commercially available blocking buffer was performed for 30 minutes at RT. Samples were incubated with primary antibody (1 : 50) for 18 hours at 4°C. An Alexa Fluor^®488-conjugated donkey polyclonal to goat IgG was used as secondary antibody at 1/100 dilution. Green color indicates GFP/Fibrolast cells, while red color indicates Ki67 positive cells, most of them are tumor cells (Abcam`s ab15580 was used for the detection).

This image is a courtesy of Hongwei Shao

• ICC/IF

AbReview25217****

Immunocytochemistry/ Immunofluorescence - Biotin Anti-GFP antibody (AB6658)

ab6658 staining GFP in rat brain cells infected with viruses containing GFP under a CMV promoter by Immunocytochemistry/ Immunofluorescence.Cells were fixed with formaldehyde, permeabilized using 0.2% Triton, blocked with 20% serum and then incubated with ab6658 at a 1 : 50 dilution for 20 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated rabbit polyclonal, used at a 1/200 dilution.

Image courtesy of Efrat Shema by Abreview.

• ELISA

Supplier Data

ELISA - Biotin Anti-GFP antibody (AB6658)

ELISA Results of Goat Anti-GFP Antibody tested against purified GFP protein. Each well was coated in duplicate with 10µg/mL of GFP protein. The starting dilution of antibody was 5μg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer and SA-HRP conjugated (high-purity, biotin-binding detection reagent).