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AB320073

Biotin Isotype Control [EPR25A]

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Biotin Isotype Control [EPR25A] Suitable for IHC-P, WB and reacts with Human samples.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Isotype Control [EPR25A] (AB320073)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Isotype Control [EPR25A] (AB320073)

IHC image of formalin-fixed paraffin-embedded human breast cancer sections* tested on a Leica BONDTM.

Sections were pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The sections were then incubated with antibody (ab317077, Rabbit monoclonal to ISG15 at 5ug/ml) or isotype control (ab320073, Rabbit IgG, at 5ug/ml) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX. The background control image is taken from an identical assay without primary antibody or isotype control.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

관련 conjugated 항체와 다양한 조성의 항체 (15)

  • Unconjugated

    Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • Carrier free

    Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free

  • Carrier free

    Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free)

  • 660 APC

    APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 519 FITC

    FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • HRP

    HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 578 PE

    PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 675 PerCP

    PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25A

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

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보완 제품

Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
  • 5
Primary Antibodies

AB202886

Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (AB202886)

  • 0
  • 0
Primary Antibodies

AB317078

Biotin Anti-Cytokeratin 18 antibody [SP69]

BOND RX™ Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Cytokeratin 18 antibody [SP69] (AB317078)

  • 0
  • 0
Primary Antibodies

AB18418

Biotin Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control

  • 0
  • 0
Primary Antibodies

AB222760

Biotin Anti-beta Actin antibody [RM112] - N-terminal - Loading Control

Recombinant

Immunocytochemistry/ Immunofluorescence - Biotin Anti-beta Actin antibody [RM112] - N-terminal - Loading Control (AB222760)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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