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AB17269

Biotin Anti-M13 antibody [E1]

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Anti-M13 antibody [E1] conjugated to Biotin - suitable for ELISA, flow cytometry, IHC, Western blot. Clone [E1] is a mouse monoclonal antibody.
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ELISA - Biotin Anti-M13 antibody [E1] (AB17269)
  • ELISA

Supplier Data

ELISA - Biotin Anti-M13 antibody [E1] (AB17269)

ELISA absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of ab17269.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

E1

Isotype

IgG3

Light chain 타입

unknown

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Enterobacteria phage M13

Applications

Flow Cyt, IHC, WB, ELISA

applications

Specificity

M13 antibody [E1] (ab17269) is specific for gp3 protein.

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대체품

Primary Antibodies

AB235228

Anti-M13 antibody

ELISA - Anti-M13 antibody (AB235228)

  • 0
  • 0

보완 제품

Primary Antibodies

AB305291

HRP Anti-M13 Bacteriophage antibody [MM05]

Recombinant

Indirect ELISA - HRP Anti-M13 Bacteriophage antibody [MM05] (AB305291)

  • 0
  • 0
Primary Antibodies

AB173828

Biotin Anti-6X His tag® antibody [HIS.H8]

Western blot - Biotin Anti-6X His tag® antibody [HIS.H8] (AB173828)

  • 0
  • 0
Primary Antibodies

AB9225

Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]

Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (AB9225)

  • 4
  • 3
Proteins & Peptides

AB7403

Streptavidin (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (AB7403)

  • 5
  • 7
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Enterobacteria phage M13": { "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/unavailable/mouse-igg2a-monoclonal-biotin-isotype-control-ab97679'>ab97679</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
Purification 관련 사항
M13 antibody [E1] (ab17269) is purified using 0.2µm sterile filter.
보관 버퍼
Preservative: 0.08% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The target 'M13' also known as M13 bacteriophage is a filamentous bacteriophage with a mass of approximately 2 MDa. This bacteriophage infects Escherichia coli bacteria and is characterized by a cylindrical shape with helical symmetry. The M13 phage expresses a protein coat made up of thousands of coat proteins including major coat protein g8p and minor proteins like g3p at its ends. These proteins interact with the bacterial surface for infection.
Biological function summary

M13 phage serves as a tool in phage display technology facilitating the identification of peptide-protein interactions. It does not actively participate in cellular processes but can be part of complex interactions in biotechnological applications. In contrast to lytic phages M13 allows for the continued survival of its host leading to its utilization in non-destructive recombinant DNA methodologies.

Pathways

M13 phage does not integrate into traditional metabolic or signaling pathways of the bacterial host. Instead it exploits the host's machinery for its replication and assembly. Not directly related to human biological pathways its use in phage display allows exploring interactions involving proteins such as antibodies or enzymes including the anti M13 HRP.

M13 phage does not directly relate to human diseases but it is essential in diagnostics and therapeutic antibody development. Researchers use phage display libraries to identify antibodies against targets involved in cancer and autoimmune diseases. The interaction between M13 and the Fc region of antibodies is exploited aiding in the precise selection of antibody fragments for therapeutic applications.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information M13

대체 명칭 보기

Bacteriophage M13, Coliphage M13, Enterobacteria phage M13, M13 virus, Phage M13
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Methods in enzymology 70:266-74 PubMed7421592

1980

Use of the double-antibody method to separate antibody bound from free ligand in radioimmunoassay.

Applications

Unspecified application

Species

Unspecified reactive species

A R Midgley,M R Hepburn
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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