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AB316948

Biotin Anti-Mast Cell Tryptase antibody [EPR9522]

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Rabbit Recombinant Monoclonal TRYB1 antibody - conjugated to Biotin. Suitable for IHC-P, WB and reacts with Human samples.

대체 명칭 보기

TPS1, TPS2, TPSB1, TPSAB1, Tryptase alpha/beta-1, Tryptase-1, Tryptase I, Tryptase alpha-1, TPS2, TPSB2, Tryptase beta-2, Tryptase-2, Tryptase II

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Mast Cell Tryptase antibody [EPR9522] (AB316948)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Mast Cell Tryptase antibody [EPR9522] (AB316948)

Negative staining of Mast Cell Tryptase in a section of formalin-fixed paraffin-embedded human normal skeletal muscle*.

Performed on a Leica BONDTM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab316948 at 0.05 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Mast Cell Tryptase antibody [EPR9522] (AB316948)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Mast Cell Tryptase antibody [EPR9522] (AB316948)

IHC image of Mast Cell Tryptase staining in a section of formalin-fixed paraffin-embedded normal human colon*.

Performed on a Leica BONDTM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab316948 at 0.05 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

관련 conjugated 항체와 다양한 조성의 항체 (6)

  • Unconjugated

    Anti-Mast Cell Tryptase antibody [EPR9522]

  • Carrier free

    Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free

  • Carrier free

    Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Mast Cell Tryptase antibody [EPR9522]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Mast Cell Tryptase antibody [EPR9522]

  • HRP

    HRP Anti-Mast Cell Tryptase antibody [EPR9522]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9522

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB323931

Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit

SimpleStep|Recombinant

Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)

  • 0
  • 0
Proteins & Peptides

AB65866

Human IgE protein (Azide free)

SDS-PAGE - Human IgE protein (Azide free) (AB65866)

  • 4
  • 2
Primary Antibodies

AB186417

Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (AB186417)

  • 5
  • 3
Secondary Antibodies

AB99806

Mouse monoclonal [B3102E8] Anti-Human IgE Fc (HRP)

Indirect ELISA - Mouse monoclonal [B3102E8] Anti-Human IgE Fc (HRP) (AB99806)

  • 4
  • 1
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.05 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed : 18854315).
See full target information TPSAB1

Additional targets

TPSB2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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