AB271279

Biotin Anti-SDF1 beta antibody

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Anti-SDF1 beta antibody conjugated to Biotin is a goat polyclonal antibody suitable for Western blot, sELISA.

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Sandwich ELISA - Biotin Anti-SDF1 beta antibody (AB271279)

sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-SDF1 beta antibody (AB271279)

Human SDF1 beta was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271279.

This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant SDF1 beta protein.

Supplier Data

Western blot - Biotin Anti-SDF1 beta antibody (AB271279)

To detect Human SDF-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.

When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Lane 1 : Marker

Lanes 2-12 : 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human SDF1 beta, respectively.

Non-reducing conditions.

All lanes:

Western blot - Biotin Anti-SDF1 beta antibody (ab271279)

Predicted band size: 11 kDa

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주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Applications

WB, sELISA

applications

Immunogen

Recombinant Fragment Protein within Human CXCL12 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P48061

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p>When used in conjunction with compatible secondary reagents, the detection limit for recombinant human SDF1 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.</p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Lyophilized

Reconstitution

Reconstitute in PBS, 0.1% BSA

Purification 테크닉

Affinity purification

보관 버퍼

Constituents: PBS, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Store in the dark

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SDF1 beta also known as CXCL12b or stromal cell-derived factor 1 beta is a small chemokine with a mass around 8 kDa. SDF1 beta plays a significant role as a signaling molecule involved in mediating various cellular responses. Expression of this chemokine occurs in a variety of tissues including spleen brain liver and skeletal muscle. Its mechanical function mainly involves binding to the CXCR4 receptor on target cells leading to the activation of signaling cascades that modulate cellular behavior.

Biological function summary

SDF1 beta participates in the regulation of immune cell trafficking and homing. As a prominent chemokine it directs leukocytes to sites where they perform their immunological duties such as inflamed or damaged tissue. Although it generally acts independently SDF1 beta can be part of a larger chemokine network collaborating with other chemokines and cytokines to orchestrate coordinated migration and positioning of immune cells.

Pathways

SDF1 beta is intricately linked with the chemokine signaling pathway and homing of hematopoietic stem cells. Activation of its pathway involves the prominent receptor CXCR4 which mediates signals that are important for the directional movement of these cells. SDF1 beta plays a central role in the hematopoiesis process where it interacts with molecules like CXCR7 which sometimes acts as a supportive player for its receptor influencing the hematopoietic stem cell niche regulation as well.

Involvement of SDF1 beta has been identified in cancer progression and inflammatory diseases like rheumatoid arthritis. In cancer it contributes to tumor growth and metastasis with CXCR4 being its main receptor partner. In rheumatoid arthritis overexpression or dysregulation of SDF1 beta influences the infiltration and retention of immune cells in the joints exacerbating inflammation. Understanding these interactions provides insights for potential therapeutic approaches targeting the SDF1 beta and CXCR4 axis in disease contexts.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils (PubMed : 18802065, PubMed : 39093700). Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis (PubMed : 8752281, PubMed : 18802065, PubMed : 39093700). Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for CXCL12/SDF-1 (PubMed : 16107333, PubMed : 19255243). Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV : ITGB3, ITGA4 : ITGB1 and ITGA5 : ITGB1 in a CXCR4-independent manner (PubMed : 29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase (PubMed : 18802065). Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins (PubMed : 16107333, PubMed : 18802065, PubMed : 19255243, PubMed : 39093700). CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase (PubMed : 18802065). Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1 (PubMed : 8752281). Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation (By similarity). Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).. SDF-1-beta(3-72). Shows a reduced chemotactic activity.. SDF-1-alpha(3-67). Shows a reduced chemotactic activity (PubMed : 14525775). Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites (PubMed : 14525775).

See full target information CXCL12

대체 명칭 보기

SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nutrients 14: PubMed35565857

2022

Piceatannol SNEDDS Attenuates Estradiol-Induced Endometrial Hyperplasia in Rats by Modulation of NF-κB and Nrf2/HO-1 Axes.

Applications

Unspecified application

Species

Unspecified reactive species

Lenah S Binmahfouz,Basma G Eid,Amina M Bagher,Rasheed A Shaik,Najlaa S Binmahfouz,Ashraf B Abdel-Naim

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-SDF1 beta antibody

Sandwich ELISA - Biotin Anti-SDF1 beta antibody (AB271279)

Western blot - Biotin Anti-SDF1 beta antibody (AB271279)

주요 정보

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

보완 제품

Assay Kits

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Reconstitution

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise