AB83145

Biotin Anti-sRANKL antibody

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Anti-sRANKL antibody conjugated to Biotin is a rabbit polyclonal antibody suitable for Western blot, sELISA.

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Sandwich ELISA - Biotin Anti-sRANKL antibody (AB83145)

sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-sRANKL antibody (AB83145)

To detect human sRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83145 is required. This biotinylated polyclonal antibody, in conjunction with an anti-RANKL (ab9957) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human sRANKL.

Supplier Data

Western blot - Biotin Anti-sRANKL antibody (AB83145)

To detect human sRANKL by Western Blot analysis ab83145 can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human sRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Image shown is non-reducing conditions; Human recombinant sRANKL protein at 250 - 0.24 ng/ml.

Lane 1 is MW marker, lanes 2-12 human recombinant sRANKL protein.

All lanes:

Western blot - Biotin Anti-sRANKL antibody (ab83145)

Predicted band size: 35 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Applications

WB, sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TNFSF11.

O14788

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p>The detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non reducing conditions.</p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.25-1 µg/mL", "sELISA-species-notes": "<p>Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human sRANKL.</p>" } } }

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특성 및 보관 정보

제형

Lyophilized

Reconstitution

Centrifuge vial prior to opening. Reconstitute in 50 µl sterile PBS containing 0.1% BSA to give a concentration of 1.0 mg/ml.

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab83145 is sterile filtered. ab83145 was purified by affinity chromatography and then biotinylated.

보관 버퍼

Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SRANKL also known as soluble receptor activator of nuclear factor kappa-B ligand is an essential protein involved in bone metabolism. It acts as a cytokine and is a member of the tumor necrosis factor (TNF) superfamily. sRANKL is derived from the membrane-bound form of RANKL by proteolytic cleavage and has a molecular weight of approximately 18 kDa. This protein is predominantly found in osteoblasts osteocytes and T-cells where it plays a critical role in physiological processes.

Biological function summary

SRANKL facilitates the differentiation and activation of osteoclasts which are cells responsible for bone resorption. It binds to its receptor RANK on osteoclast precursors promoting their maturation into fully functional osteoclasts. This binding process involves complex formation and interaction with another molecule called osteoprotegerin (OPG) which acts as a decoy receptor regulating sRANKL activity and therefore controlling bone resorption.

Pathways

SRANKL plays a fundamental role in the RANK/RANKL/OPG signaling pathway which is important for maintaining bone homeostasis. This pathway regulates the balance between bone formation and resorption. It also interacts with NF-kappaB signaling a pathway that influences inflammation and immune response. sRANKL through these pathways closely connects with proteins such as TRAF6 which mediates downstream signaling leading to osteoclastogenesis.

Alterations in the sRANKL pathway impact bone-related diseases such as osteoporosis and rheumatoid arthritis. In osteoporosis increased levels of sRANKL enhance osteoclast activity leading to excessive bone resorption and thereby weakening bones. In rheumatoid arthritis the interaction between sRANKL and RANK promotes inflammation and joint destruction. This protein is linked to OPG as variations in their ratio influence disease development and progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed : 22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).

See full target information TNFSF11

대체 명칭 보기

CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental dermatology 32:403-412 PubMed36457234

2022

CCL6 promotes M2 polarization and inhibits macrophage autophagy by activating PI3-kinase/Akt signalling pathway during skin wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Feng,Yu Ji,Ce Zhang,Tingting Jin,Jingyu Li,Jincai Guo

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-sRANKL antibody

Sandwich ELISA - Biotin Anti-sRANKL antibody (AB83145)

Western blot - Biotin Anti-sRANKL antibody (AB83145)

주요 정보

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Assay Kits

Sample Prep & Detection Kits

Primary Antibodies

Proteins & Peptides

Assay Kits

Reactivity 정보

특성 및 보관 정보

제형

Reconstitution

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise