Biotin Anti-VSV-G tag antibody

• WB

AbReview20545****

Western blot - Biotin Anti-VSV-G tag antibody (AB34774)

Both lanes are ab34774 at a 1/500 dilution plus 20μg of whole cell lysate prepared from HeLa cells transfected with VSV-G. A Streptavidin HRP conjugate was used at a 1/2000 dilution.
Primary antibody incubated for 16 hours at 4°C.
Blocking step performed using 5% milk for 1 hour at 23°C.

All lanes:

Western blot - Biotin Anti-VSV-G tag antibody (ab34774)

Predicted band size: 57.5 kDa

Observed band size: 60 kDa

true

Exposure time: 1min

Image courtesy of an anonymous Abreview.

• ICC/IF

AbReview20628****

Immunocytochemistry/ Immunofluorescence - Biotin Anti-VSV-G tag antibody (AB34774)

ab34774 staining VSV-G tag in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.025% Triton-X in PBS and then blocked using 5% serum for 1 hour. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat polyclonal conjugated to Alexa Fluor® 488 (green) used undiluted.

Image courtesy of an anonymous Abreview.

• ELISA

AbReview20633****

ELISA - Biotin Anti-VSV-G tag antibody (AB34774)

ab34774 detecting the VSV-G tag in HeLa cells expressing VSV-G by direct ELISA. Samples were blocked with 5% BSA for 1 hour at 23°C and incubated with the primary antibody (1/3000 in blocking buffer) for 16 hours at 4°C. An undiluted streptavidin-alkaline phosphatase conjugate polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview