Anti-Blooms Syndrome Protein Blm antibody

• WB

Unknown

Western blot - Anti-Blooms Syndrome Protein Blm antibody (AB476)

Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band.

Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band.

All lanes:

Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab476)

Predicted band size: 159 kDa

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• IP

Unknown

Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (AB476)

BLAP75 physically interacts with BLM and Topo IIIa. Purified BLM was incubated with purified Topo IIIa and the reaction mixture was subjected to Immunoprecipitation with ab476. The reaction supernatant (S), wash (W), and eluate (E) were analyzed by SDS-PAGE, GST-BLAP75 or GST alone was incubated with BLM. Protein complexes were captured on glutathione-Sepharose beads, followed by SDS-PAGE.

All lanes:

Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab476)

Predicted band size: 159 kDa

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Image from S Raynard et al, J Biol Chem 281:13861-4 (2006), Fig 2.

• WB

CiteAb

Western blot - Anti-Blooms Syndrome Protein Blm antibody (AB476)

Blooms Syndrome Protein Blm western blot using anti-Blooms Syndrome Protein Blm antibody ab476. Publication image and figure legend from Chaudhury, I., Sareen, A., et al., 2013, Nucleic Acids Res, PubMed 23658231.

ab476 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab476 please see the product overview.

Human FANCD2 and BLM act in one pathway to mediate replication fork restart and suppression of new origin firing. (A) Human cell types used for DNA fiber analysis : wild type (PD20 + D2, siControl), FANCD2 deficient (PD20, siControl), BLM deficient (PD20 + D2, siBLM) and FANCD2/BLM double deficient (PD20, siBLM). (B) Images of DNA fibers with a schematic of defining sites of replication. Red tracts : DigU; green tracts : BioU. (C) FANCD2 and BLM act in a common pathway to mediate replication fork restart after replication blockade. The efficiency of replication restart in wild-type, FANCD2-deficient, BLM-deficient and FANCD2/BLM double-deficient cells was measured as the number of restarted replication forks after APH-mediated fork stalling (DigU→BioU tracts), compared with the total number of DigU-labeled tracts (DigU plus DigU→BioU). (D) FANCD2 and BLM act in concert to suppress new origin firing during replication blockade. The number of new sites of replication originating during the 40 min recovery period after APH treatment was compared between wild-type, FANCD2-deficient, BLM-deficient and FANCD2/BLM double-deficient cells. New origins of replication were measured as the number of green-only (BioU) tracts per unit length. ***p < 0.0001.

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