AB78422

Anti-BMPR2 antibody [MM0060-9A10]

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(13 제품이 사용된 논문 )

Mouse Monoclonal BMPR2 antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BMPR2.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR2 antibody [MM0060-9A10] (AB78422)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR2 antibody [MM0060-9A10] (AB78422)

ab78422 at 1/200 dilution, staining BMPR2 in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

Flow Cytometry - Anti-BMPR2 antibody [MM0060-9A10] (AB78422)

Flow Cyt

Unknown

Flow Cytometry - Anti-BMPR2 antibody [MM0060-9A10] (AB78422)

Overlay histogram showing HepG2 cells stained with ab78422 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78422, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0060-9A10

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human BMPR2.

Q13873

Specificity

ab78422 detects BMPR2. No cross reactivity was found to BMPR1A and BMPR1B.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^5 Cells", "FlowCyt-species-notes": "(methanol or paraformaldehyde fixed cells) <a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "We recommend to use Proteinase K for the enzymatic antigen retrieval." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate buffered saline (PBS).

보관 버퍼

Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Can also mediate signaling through the activation of the p38MAPK cascade (PubMed : 12045205). Binds to BMP7, BMP2 and, less efficiently, BMP4. Binding is weak but enhanced by the presence of type I receptors for BMPs. Mediates induction of adipogenesis by GDF6. Promotes signaling also by binding to activin A/INHBA (PubMed : 24018044).

See full target information BMPR2

대체 명칭 보기

PPH1, BMPR2, Bone morphogenetic protein receptor type-2, BMP type-2 receptor, BMPR-2, Bone morphogenetic protein receptor type II, BMP type II receptor, BMPR-II

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제품이 사용된 논문 (13)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 24: PubMed37686014

2023

CRISPR-Cas9 Library Screening Identifies Novel Molecular Vulnerabilities in -Rearranged Acute Lymphoblastic Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Pauline Schneider,Priscilla Wander,Susan T C J M Arentsen-Peters,Kirsten S Vrenken,Dedeke Rockx-Brouwer,Fabienne R S Adriaanse,Veerle Hoeve,Irene Paassen,Jarno Drost,Rob Pieters,Ronald W Stam

Scientific reports 12:16310 PubMed36175474

2022

BMP2 promotes lung adenocarcinoma metastasis through BMP receptor 2-mediated SMAD1/5 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Kuei Wu,Man-Ting Wei,Hung-Chang Wu,Cheng-Lin Wu,Cheng-Ju Wu,Hungjiun Liaw,Wen-Pin Su

Bioengineered 13:8538-8547 PubMed35331081

2022

Lysine (K)-specific demethylase 5C regulates the incidence of severe preeclampsia by adjusting the expression of bone morphogenetic protein-7.

Applications

Unspecified application

Species

Unspecified reactive species

Xu-Feng Shi,Zhan Zhang,Hai-Ying Wu,Yu Wang,Ai-Min Chang,Jun-Jun Gao,Kan Liu,Wan-Yu Song,Li Wang,Huan-Ping Wang

Cell death & disease 10:261 PubMed30886151

2019

Osteosarcoma cell intrinsic PD-L2 signals promote invasion and metastasis via the RhoA-ROCK-LIMK2 and autophagy pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Ren,Bingxin Zheng,Yi Huang,Shidong Wang,Xing Bao,Kuisheng Liu,Wei Guo

Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 29:447-454 PubMed29142464

2017

BMPR2 and HIF1- overexpression in resected osteosarcoma correlates with distant metastasis and patient survival.

Applications

Unspecified application

Species

Unspecified reactive species

Shidong Wang,Tingting Ren,Yi Huang,Xing Bao,Kunkun Sun,Danhua Shen,Wei Guo

Oncotarget 8:58625-58641 PubMed28938584

2017

BMPR2 promotes invasion and metastasis via the RhoA-ROCK-LIMK2 pathway in human osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shidong Wang,Tingting Ren,Guangjun Jiao,Yi Huang,Xing Bao,Fan Zhang,Kuisheng Liu,Bingxin Zheng,Kunkun Sun,Wei Guo

Science signaling 9:ra58 PubMed27273096

2016

Augmented noncanonical BMP type II receptor signaling mediates the synaptic abnormality of fragile X syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Risa Kashima,Sougata Roy,Manuel Ascano,Veronica Martinez-Cerdeno,Jeanelle Ariza-Torres,Sunghwan Kim,Justin Louie,Yao Lu,Patricio Leyton,Kenneth D Bloch,Thomas B Kornberg,Paul J Hagerman,Randi Hagerman,Giorgio Lagna,Akiko Hata

Proceedings of the National Academy of Sciences of 112:E6882-8 PubMed26621735

2015

Identifying an ovarian cancer cell hierarchy regulated by bone morphogenetic protein 2.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Jung Choi,Patrick N Ingram,Kun Yang,Lan Coffman,Mangala Iyengar,Shoumei Bai,Dafydd G Thomas,Euisik Yoon,Ronald J Buckanovich

Experimental biology and medicine (Maywood, N.J.) 240:1580-9 PubMed25854878

2015

Featured Article: microRNA-125a in pulmonary hypertension: Regulator of a proliferative phenotype of endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lars C Huber,Silvia Ulrich,Caroline Leuenberger,Max Gassmann,Johannes Vogel,Leonardo Glutz von Blotzheim,Rudolf Speich,Malcolm Kohler,Matthias Brock

Cell death & disease 5:e1571 PubMed25501832

2014

BMPR2 inhibition induced apoptosis and autophagy via destabilization of XIAP in human chondrosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

G Jiao,W Guo,T Ren,Q Lu,Y Sun,W Liang,C Ren,K Yang,K Sun

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BMPR2 antibody [MM0060-9A10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR2 antibody [MM0060-9A10] (AB78422)

Flow Cytometry - Anti-BMPR2 antibody [MM0060-9A10] (AB78422)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (13)

Product promise