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AB99891

Anti-BOB1 antibody [SP92]

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Anti-BOB1 antibody [SP92] (ab99891) is a rabbit monoclonal antibody detecting BOB1 in Western Blot, IHC-P. Suitable for Human.

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- Trusted since 2010
3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (AB99891)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (AB99891)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling BOB1 with Purified ab99891 at 1/100 dilution (0.96 μg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 20mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody.

Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (AB99891)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (AB99891)

Staining of Human BOB1 in a Formalin/PFA-fixed paraffin-embedded section of Human Tonsil using ab99891 at a dilution of 1/100.

Western blot - Anti-BOB1 antibody [SP92] (AB99891)
  • WB

Unknown

Western blot - Anti-BOB1 antibody [SP92] (AB99891)

All lanes:

Western blot - Anti-BOB1 antibody [SP92] (ab99891) at 1/100 dilution

All lanes:

lysate prepared from Ramos cells

Predicted band size: 27 kDa

Observed band size: 35 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (8)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP92

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB3426

Anti-BOB1 antibody

Western blot - Anti-BOB1 antibody (AB3426)

  • 0
  • 0
Primary Antibodies

AB52103

Anti-BOB1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody (AB52103)

  • 0
  • 0
Primary Antibodies

AB92315

Anti-BOB1 antibody [EPR3299]

RabMAb|Recombinant

Western blot - Anti-BOB1 antibody [EPR3299] (AB92315)

  • 0
  • 0
Primary Antibodies

AB203110

Anti-BOB1 antibody [EPR17685]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] (AB203110)

  • 0
  • 0
Primary Antibodies

AB76010

Anti-BOB1 antibody [EP3300]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-BOB1 antibody [EP3300] (AB76010)

  • 0
  • 0

보완 제품

Primary Antibodies

AB16662

Anti-ErbB2 / HER2 antibody [SP3]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (AB16662)

  • 5
  • 2
Primary Antibodies

AB110641

Anti-BRG1 antibody [EPNCIR111A]

20ul selling size|BOND RX™ Validated|Recombinant|KO Validated|Advanced Validation|RabMAb

ChIC/CUT&RUN sequencing - Anti-BRG1 antibody [EPNCIR111A] (AB110641)

  • 5
  • 10
Primary Antibodies

AB16702

Anti-CD23 antibody [SP23]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry - Anti-CD23 antibody [SP23] (AB16702)

  • 0
  • 0
Primary Antibodies

AB219650

Anti-PRAME antibody [EPR20330]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAME antibody [EPR20330] (AB219650)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.</p>" } } }
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제품 세부 정보

What is this antibody validated in?
Anti-BOB1 antibody [SP92] (ab99891) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

Collaboration
Anti-BOB1 [SP92] (ab99891) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Other related products
We have a range of other formats of antibody clone [SP92] also available for your convenience: ab99891, Carrier free - ab239921, ab279436, Alexa Fluor® 488 - ab320611, Alexa Fluor® 647 - ab320612, Alexa Fluor® 555 - ab320613, Alexa Fluor® 594 - ab320614, PE - ab320615, APC - ab320616, Alexa Fluor® 750 - ab321780

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
Purification 관련 사항
Purified from TCS by protein A/G.
보관 버퍼
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed : 7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed : 7779176). It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed : 7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed : 7623806, PubMed : 7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).
See full target information POU2AF1

대체 명칭 보기

BOB1, OBF1, POU2AF1, POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Modern pathology : an official journal of the Unit 30:1144-1151 PubMed28548127

2017

Mismatch repair deficiency commonly precedes adenoma formation in Lynch Syndrome-Associated colorectal tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shigeki Sekine,Taisuke Mori,Reiko Ogawa,Masahiro Tanaka,Hiroshi Yoshida,Hirokazu Taniguchi,Takeshi Nakajima,Kokichi Sugano,Teruhiko Yoshida,Mamoru Kato,Eisaku Furukawa,Atsushi Ochiai,Nobuyoshi Hiraoka
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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