Anti-Bovine Herpesvirus 1- gE antibody [1B5]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (AB325992)

Immunofluorescence labeling Bovine Herpesvirus type 1 (BoHV1) in positive control (about 30% MDBK cells with virus infection). Primary antibody ab325992 at 4 ug/mL; Secondary antibody Goat anti-mouse IgG (H+L) FITC conjugated at 1 : 1000 (1 ug/mL) dilution (green); DAPI : Nuclear counterstain (blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (AB325992)

Immunofluorescence labeling Bovine Herpesvirus type 1 (BoHV1) in negative control (about 30% MDBK cells without virus infection). Primary antibody ab325992 at 4 ug/mL; Secondary antibody Goat anti-mouse IgG (H+L) FITC conjugated at 1 : 1000 (1 ug/mL) dilution (green); DAPI : Nuclear counterstain (blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (AB325992)

Immunofluorescence labeling Bovine Herpesvirus type 1 (BoHV1) in positive control (about 30% MDBK cells with virus infection). Primary antibody ab325992 at 4 ug/mL; Secondary antibody Goat anti-mouse IgG (H+L) FITC conjugated at 1 : 1000 (1 ug/mL) dilution (green); DAPI : Nuclear counterstain (blue).

• WB

Supplier Data

Western blot - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (AB325992)

Western Blot Assay : Primary antibody ab325992 at 0.1 ug/mL for 1 hr at room temperature, followed by a Goat anti-mouse IgG secondary at 1 : 2000 dilution for 1 hr at room temperature. Lane 1 : MW. Lane 2 : BoHV1 gE 1ug. Lane 3 : BoHV1 gE 0.1ug. Lane 4 : BoHV1 gB 1ug.

All lanes:

Western blot - Anti-Bovine Herpesvirus 1- gE antibody [1B5] (ab325992) at 0.1 µg/mL

Lane 2:

BoHV1 gE at 1 µg

Lane 3:

BoHV1 gE at 0.1 µg

Lane 4:

BoHV1 gB at 1 µg

Secondary

All lanes:

Goat anti-mouse IgG at 1/2000 dilution

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