AB980

Anti-Bovine Papilloma Virus E2 antibody [1E4]

Name: Anti-Bovine Papilloma Virus E2 antibody [1E4]
Brand: Abcam Limited
SKU: AB980
Rating: 5 (2 reviews)

(2 리뷰들)

(5 제품이 사용된 논문 )

Mouse Monoclonal VE2 antibody. Suitable for WB, IP, ICC/IF and reacts with Recombinant full length protein - Bovine papillomavirus, Bovine papillomavirus samples. Cited in 5 publications.

1 이미지

Supplier Data

Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (AB980)

All lanes:

Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)

Lane 1:

Purified recombinant Bovine Papillomavirus E2 protein at 15000 µg

Lane 2:

Purified recombinant Bovine Papillomavirus E2 protein at 10000 µg

Lane 3:

Purified recombinant Bovine Papillomavirus E2 protein at 5000 µg

Lane 4:

Purified recombinant Bovine Papillomavirus E2 protein at 1000 µg

Predicted band size: 45 kDa

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

1E4

Isotype

IgG1

Carrier free

Reacts with

Bovine papillomavirus

Applications

ICC/IF, IP, WB

applications

Epitope

Not Determined.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bovine papillomavirus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "To isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "1hr, RT. Dilute in a diluent containing 1% BSA in PBS." }, "Recombinant full length protein - Bovine papillomavirus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "1hr, RT. Dilute in a diluent containing 2% non-fat dry milk in TBST. (Background can be reduced by adding NaCl up to a concentration of 1.5 M.)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Constituents: 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Bovine Papilloma Virus E2 (BPV E2) is an important regulatory protein involved in the lifecycle of the Bovine Papilloma Virus. It also goes by alternate names such as E2 protein or Papilloma E2 antigen. The E2 protein has a mass of approximately 40 kDa. It is expressed during the early phase of viral infection within the nucleus of infected cells. BPV E2 plays a mechanical role by binding to DNA at specific sequences facilitating viral replication and transcription.

Biological function summary

BPV E2 functions as a transcription factor that regulates the expression of viral genes. It is part of a larger complex with other viral proteins such as E1 with which it cooperates to initiate viral replication. E2’s control over viral gene expression allows it to modulate the virus's ability to replicate and persist in host cells influencing the viral lifecycle and maintenance.

Pathways

BPV E2 is integral in the regulation of pathways related to viral replication and transcription. It is closely involved in the papillomavirus replication pathway interacting with proteins such as E1 to initiate DNA replication. Another relevant pathway includes transcriptional regulation where E2 controls the expression of various viral genes contributing to papillomavirus pathogenesis.

BPV E2 is connected to bovine papillomatosis a condition characterized by wart formation in cattle. This disorder arises due to the uncontrolled proliferation of infected cells mediated by the viral replication facilitated by BPV E2. Its interaction with the E1 protein is essential for the viral replication that leads to disease progression. Understanding E2 and its role offers potential insights into therapeutic targets using bovine antibodies and possibly developing anti-papilloma strategies in bovine species.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.

See full target information E2

대체 명칭 보기

Regulatory protein E2, E2

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Acta neuropathologica 138:457-476 PubMed31011859

2019

Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Beatriz Garcia-Diaz,Corinne Bachelin,Fanny Coulpier,Gaspard Gerschenfeld,Cyrille Deboux,Violetta Zujovic,Patrick Charnay,Piotr Topilko,Anne Baron-Van Evercooren

Molecular and cellular biology 37: PubMed28069738

2017

FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Takayoshi Yamauchi,Masaaki Nishiyama,Toshiro Moroishi,Atsuki Kawamura,Keiichi I Nakayama

Endocrinology 157:3682-95 PubMed27501182

2016

Type 2 Deiodinase Disruption in Astrocytes Results in Anxiety-Depressive-Like Behavior in Male Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara M L C Bocco,João Pedro Werneck-de-Castro,Kelen C Oliveira,Gustavo W Fernandes,Tatiana L Fonseca,Bruna P P Nascimento,Elizabeth A McAninch,Esther Ricci,Zsuzsanna Kvárta-Papp,Csaba Fekete,Maria Martha Bernardi,Balázs Gereben,Antonio C Bianco,Miriam O Ribeiro

Nature medicine 18:436-40 PubMed22366949

2012

Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL.

Applications

ChIP

Species

Unspecified reactive species

Giusy Della Gatta,Teresa Palomero,Arianne Perez-Garcia,Alberto Ambesi-Impiombato,Mukesh Bansal,Zachary W Carpenter,Kim De Keersmaecker,Xavier Sole,Luyao Xu,Elisabeth Paietta,Janis Racevskis,Peter H Wiernik,Jacob M Rowe,Jules P Meijerink,Andrea Califano,Adolfo A Ferrando

Journal of virology 73:4670-7 PubMed10233926

1999

Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

R Kurg,J Parik,E Juronen,T Sedman,A Abroi,I Liiv,U Langel,M Ustav

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bovine Papilloma Virus E2 antibody [1E4]

Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (AB980)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Epitope

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Sample Prep & Detection Kits

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise