AB259260

Anti-Brachyury / Bry antibody [54]

BOND RX™ Validated
Recombinant
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Rabbit Recombinant Monoclonal Brachyury / Bry antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

T, TBXT, T-box transcription factor T, Brachyury protein, Protein T

5 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Brachyury / Bry antibody [54] (AB259260)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Brachyury / Bry antibody [54] (AB259260)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized MUG-Chor1 cells labelling Brachyury / Bry with ab259260 at 1/50 (20.28 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MUG-Chor1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (AB259260)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (AB259260)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Brachyury / Bry with ab259260 at 1/5000 (0.203 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining on human lung (PMID : 23456319). The section was incubated with ab259260 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with .

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (AB259260)

Immunohistochemical analysis of paraffin-embedded Human chordoma tissue labeling Brachyury / Bry with ab259260 at 1/5000 (0.203 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Mainly nuclear staining on human chordoma. The section was incubated with ab259260 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with .

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Lab

Immunoprecipitation - Anti-Brachyury / Bry antibody [54] (AB259260)

Brachyury / Bry was immunoprecipitated from 0.35 mg MUG-Chor1 (human sacral bone chordoma), whole cell lysate 10ug with ab259260 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259260 at 1/2000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/10000 dilution.

Lane 1 : MUG-Chor1 (human sacral bone chordoma), whole cell lysate 10ug

Lane 2 : ab259260 IP in MUG-Chor1 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab282459 in MUG-Chor1 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds

All lanes:

Immunoprecipitation - Anti-Brachyury / Bry antibody [54] (ab259260)

Predicted band size: 47 kDa

false

Lab

Western blot - Anti-Brachyury / Bry antibody [54] (AB259260)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 10 seconds

All lanes:

Western blot - Anti-Brachyury / Bry antibody [54] (ab259260) at 1/5000 dilution

All lanes:

MUG-Chor1 (human sacral bone chordoma), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 47 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Brachyury protein also known as Bry or T acts as a transcription factor and belongs to the T-box family of genes. It consists of about 435 amino acids giving it an estimated mass of around 47 kDa. Brachyury plays a critical role in the development of the posterior mesoderm and nucleus pulposus during embryogenesis. It is strongly expressed in the notochord mesoderm and some tumor tissues. The protein's presence can be detected using specific assays such as Brachyury IHC (immunohistochemistry) and Brachyury stain which help identify Brachyury marker expression in both normal and pathological states.

Biological function summary

The Brachyury protein influences the morphogenetic movement in mesoderm development. It directly interacts with specific DNA sequences to regulate the transcription of target genes. Brachyury often operates as part of a larger transcriptional complex that manages the differentiation and migration of cells during embryogenesis. The gene's expression indicates mesodermal differentiation and is important for cell fate decisions within developing embryos.

Pathways

Brachyury plays a significant role in the Wnt signaling and the TGF-beta pathways. These pathways are essential in controlling cell proliferation and differentiation. Brachyury directly impacts the regulation of these pathways influencing other proteins like beta-catenin in Wnt signaling. This interconnectedness suggests its broader role in cellular communication and development processes potentially affecting gene expression across these pathways.

Brachyury has been associated with various cancers such as chordoma and other related tumors. Chordoma specifically involves the notochord where Brachyury marks the disease's presence. Other proteins like E-cadherin interact with Brachyury in tumor progression and metastasis. Understanding Brachyury's function helps in diagnosing and potentially targeting treatment options for these ailments.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site.

See full target information TBXT

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biochemistry and biophysics reports 41:101907 PubMed39830524

2025

Effect of β-catenin on hypoxia induced epithelial mesenchymal transition in HK-2 cells by regulating Brachyury.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Sun,Haihui Yang,Binying Min,Yongfu Li,Jun Wang,Mo Chen,Diping Yu,Wenjuan Sun

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Brachyury / Bry antibody [54]

Immunocytochemistry/ Immunofluorescence - Anti-Brachyury / Bry antibody [54] (AB259260)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (AB259260)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [54] (AB259260)

Immunoprecipitation - Anti-Brachyury / Bry antibody [54] (AB259260)

Western blot - Anti-Brachyury / Bry antibody [54] (AB259260)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise