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AB326635

Anti-BRAF (phospho T401) antibody [JJ08-72]

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Rabbit Recombinant Monoclonal BRAF phospho T401 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples.
2 이미지
Western blot - Anti-BRAF (phospho T401) antibody [JJ08-72] (AB326635)
  • WB

Supplier Data

Western blot - Anti-BRAF (phospho T401) antibody [JJ08-72] (AB326635)

All lanes : Primary antibody at 1/1000 dilution
Lane 1 : K562 whole cell lysates
Lane 2 : C6 whole cell lysates
Lane 3 : 3T3 whole cell lysates
Lane 4 : Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 84 kDa
Observed band size : 84 kDa

Exposure time : 8 seconds

All lanes:

Western blot - Anti-BRAF (phospho T401) antibody [JJ08-72] (ab326635) at 1/1000 dilution

Lane 1:

K562 whole cell lysates at 20 µg

Lane 2:

C6 whole cell lysates at 20 µg

Lane 3:

3T3 whole cell lysates at 20 µg

Lane 4:

Hela whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

false

Exposure time: 8s

Western blot - Anti-BRAF (phospho T401) antibody [JJ08-72] (AB326635)
  • WB

Supplier Data

Western blot - Anti-BRAF (phospho T401) antibody [JJ08-72] (AB326635)

All lanes : Primary antibody at 1/1000 dilution
Lane 1 : PC12 whole cell lysates
Lane 2 : PC12 treated with 200nM TPA for 30 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 84 kDa
Observed band size : 84kDa

Exposure time : 7 seconds

All lanes:

Western blot - Anti-BRAF (phospho T401) antibody [JJ08-72] (ab326635) at 1/100 dilution

Lane 1:

PC12 whole cell lysates at 20 µg

Lane 2:

PC12 treated with 200nM TPA for 30 minutes whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

false

Exposure time: 7s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

JJ08-72

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB124794

Anti-BRAF (phospho S729) antibody [EPR2207]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-BRAF (phospho S729) antibody [EPR2207] (AB124794)

  • 0
  • 0
Primary Antibodies

AB33899

Anti-BRAF antibody [EP152Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] (AB33899)

  • 4
  • 12
Primary Antibodies

AB52894

Anti-EGFR antibody [EP38Y]

BOND RX™ Validated|KO Validated|RabMAb|Lab Essentials|20ul selling size|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (AB52894)

  • 5
  • 24
Primary Antibodies

AB313793

Anti-BRAF (phospho S446) antibody [EPR28069-63]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-BRAF (phospho S446) antibody [EPR28069-63] (AB313793)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.05% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).
See full target information BRAF pT401

대체 명칭 보기

BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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