Anti-BRD3 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody (AB264294)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling BRD3 with ab264294 at 1/100 dilution. Detection : DAB.

• IP

Supplier Data

Immunoprecipitation - Anti-BRD3 antibody (AB264294)

BRD3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264294 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab264294 at 1 μg/ml.

Lane 1 : ab264294 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 3 secs.

All lanes:

Immunoprecipitation - Anti-BRD3 antibody (ab264294)

Predicted band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-BRD3 antibody (AB264294)

4-8% SDS-PAGE.

All lanes:

Western blot - Anti-BRD3 antibody (ab264294) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Secondary

All lanes:

Goat anti-Rabbit Light Chain HRP Conjugate

Predicted band size: 80 kDa

false

Exposure time: 30s