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AB134061

Anti-BST2/Tetherin antibody [EPR5648]

4

(5 리뷰들)

|

(6 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal BST2/Tetherin antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

대체 명칭 보기

CD317, Bone marrow stromal antigen 2, BST-2, HM1.24 antigen, Tetherin, BST2

4 이미지
Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)
  • WB

Unknown

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)

All lanes:

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 28-40 kDa

false

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)
  • WB

Lab

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)

Blocking/Diluting buffer and concentration 5% NFDM/TBST

This antibody detects a non-specific band at 65 KDa.

This image was produced using unpurified antibody.

All lanes:

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

All lanes:

Human fetal spleen whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 30 kDa

false

Exposure time: 3min

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)
  • WB

Lab

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)

ab134061 was shown to react with BST2 in wild-type A-431 cells in western blot. Loss of signal was observed when BST2 knockout cell line ab261907 (knockout cell lysate ab261713) was used. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134061 overnight at 4°C at a 1 in 1000 dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor® 680) at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.

All lanes:

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

Lane 1:

Wild-type A431 cell lysate at 40 µg

Lane 2:

BST2 knockout A431 cell lysate at 40 µg

Lane 2:

Western blot - Human BST2 (Tetherin) knockout A-431 cell line (<a href='/ko/products/cell-lines/human-bst2-tetherin-knockout-a-431-cell-line-ab261907'>ab261907</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 40 µg

Lane 4:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 40 µg

Predicted band size: 20 kDa

Observed band size: 30 kDa

false

Exposure time: 20min

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)
  • WB

Unknown

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)

This image was produced using unpurified antibody.

All lanes:

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

Lane 1:

U937 lysate at 10 µg

Lane 2:

Molt-4 lysate at 10 µg

Lane 3:

Human spleen lysate at 10 µg

Lane 4:

HeLa lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 20 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5648

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

For Mouse and Rat species, we have preliminary internal testing data to indicate this antibody may not react. Please contact us for more information.

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대체품

Primary Antibodies

AB243561

Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free (AB243561)

  • 0
  • 0
Primary Antibodies

AB243229

Anti-BST2/Tetherin antibody [EPR20202-169]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] (AB243229)

  • 0
  • 0
Primary Antibodies

AB243230

Anti-BST2/Tetherin antibody [EPR20202-150]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-150] (AB243230)

  • 0
  • 0
Primary Antibodies

AB243563

Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free (AB243563)

  • 0
  • 0

보완 제품

Assay Kits

AB231931

Human BST2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human BST2 ELISA Kit (AB231931)

  • 0
  • 0
Primary Antibodies

AB184787

Anti-Caspase-3 antibody [EPR18297]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [EPR18297] (AB184787)

  • 3
  • 3
Primary Antibodies

AB32536

Anti-NF-kB p65 antibody [E379]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] (AB32536)

  • 4
  • 15
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB179515

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)

  • 3
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Stable for 12 months at -20°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted (PubMed : 18200009, PubMed : 18342597, PubMed : 19036818, PubMed : 19879838, PubMed : 20019814, PubMed : 20399176, PubMed : 20419159, PubMed : 20940320, PubMed : 21529378, PubMed : 22520941). Its target viruses belong to diverse families, including retroviridae : human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason-Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae : hepatitis C virus (HCV), filoviridae : ebola virus (EBOV) and marburg virus (MARV), arenaviridae : lassa virus (LASV) and machupo virus (MACV), herpesviridae : kaposis sarcoma-associated herpesvirus (KSHV), rhabdoviridae : vesicular stomatitis virus (VSV), orthomyxoviridae : influenza A virus, paramyxoviridae : nipah virus, and coronaviridae : SARS-CoV (PubMed : 18200009, PubMed : 18342597, PubMed : 19179289, PubMed : 19879838, PubMed : 20399176, PubMed : 20419159, PubMed : 20686043, PubMed : 20943977, PubMed : 21529378, PubMed : 21621240, PubMed : 22520941, PubMed : 26378163, PubMed : 31199522). Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration (PubMed : 22065321). Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection (PubMed : 19564354, PubMed : 26172439). Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. Isoform 1 and isoform 2 are both effective viral restriction factors but have differing antiviral and signaling activities (PubMed : 23028328, PubMed : 26172439). Isoform 2 is resistant to HIV-1 Vpu-mediated degradation and restricts HIV-1 viral budding in the presence of Vpu (PubMed : 23028328, PubMed : 26172439). Isoform 1 acts as an activator of NF-kappa-B and this activity is inhibited by isoform 2 (PubMed : 23028328).
See full target information BST2
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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cells 10: PubMed33800686

2021

Parkinson's Disease Causative Mutation in Vps35 Disturbs Tetherin Trafficking to Cell Surfaces and Facilitates Virus Spread.

Applications

Unspecified application

Species

Unspecified reactive species

Yingzhuo Ding,Yan Li,Gaurav Chhetri,Xiaoxin Peng,Jing Wu,Zejian Wang,Bo Zhao,Wenjuan Zhao,Xueyi Li

Cell reports 26:3257-3271.e8 PubMed30893599

2019

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Applications

Unspecified application

Species

Unspecified reactive species

Marielle Balzano,Maria De Grandis,Thien-Phong Vu Manh,Lionel Chasson,Florence Bardin,Anne Farina,Arnauld Sergé,Ghislain Bidaut,Pierre Charbord,Léonard Hérault,Anne-Laure Bailly,Amandine Cartier-Michaud,Annie Boned,Marc Dalod,Estelle Duprez,Paul Genever,Mark Coles,Marc Bajenoff,Luc Xerri,Michel Aurrand-Lions,Claudine Schiff,Stéphane J C Mancini

Cancer research 79:2220-2231 PubMed30890618

2019

CD317 Activates EGFR by Regulating Its Association with Lipid Rafts.

Applications

Unspecified application

Species

Unspecified reactive species

Guizhong Zhang,Xin Li,Qian Chen,Junxin Li,Qingguo Ruan,Youhai H Chen,Xiaolu Yang,Xiaochun Wan

Journal of molecular biology 430:3111-3128 PubMed30131116

2018

DDX17 Specifically, and Independently of DDX5, Controls Use of the HIV A4/5 Splice Acceptor Cluster and Is Essential for Efficient Replication of HIV.

Applications

Unspecified application

Species

Unspecified reactive species

Nyaradzai Sithole,Claire A Williams,Aisling M Vaughan,Julia C Kenyon,Andrew M L Lever

Virology 513:11-16 PubMed29028477

2017

Modulation of the NF-κB signaling pathway by the HIV-2 envelope glycoprotein and its incomplete BST-2 antagonism.

Applications

WB

Species

Unspecified reactive species

François E Dufrasne,Mara Lucchetti,Anandi Martin,Emmanuel André,Géraldine Dessilly,Benoit Kabamba,Patrick Goubau,Jean Ruelle

Journal of experimental & clinical cancer research 35:117 PubMed27444183

2016

CD317 Promotes the survival of cancer cells through apoptosis-inducing factor.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Guizhong Zhang,Qian Chen,Yingxue Lin,Junxin Li,Qingguo Ruan,Youhai Chen,Guang Yu,Xiaochun Wan
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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